Using Leaf Sections to Identify Different Salt Tolerance Potential of Poplar Plants

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  • 1College of Life Sciences, Tianjin Normal University, Tianjin 300384, China;

    2Tianjin Research Center of AgriculturalBio-technology, Tianjin 300192, China;

    3Institute of Genetics and Developmental Biology,Chinese Academy of Sciences, Beijing 100101, China

Received date: 2010-10-08

  Revised date: 2011-01-21

  Online published: 2011-05-26

Abstract

Plant dry weight and changes in Na+ and K+ contents in leaves were investigated in 1-year-old cuttings from the receptor poplar 107 (Populus × euramericana ‘Neva’) and transgenic poplar 18-1 after cuttings were cultured in Hoagland solution with different NaCl concentrations. The growth and rooted percentage of 18-1 was higher than that of 107 with 0.6%NaCl, and the accumulation of Na+ in 18-1 leaves was about 1.45 times higher than that in 107. Physiological characteristics in leaf sections from 107 and 18-1 under NaCl stress were determined. Leaf sections from 18-1 displayed lower chlorophyll decreasing speed and relative electrical conductivity than that from 107. When sections of young poplar leaves were plated on MS medium with 1.2%NaCl, leaf sections from 107 stopped growing, but those from 18-1 grew continuously and their length increased nearly 50% after 7 days. Thus, salt tolerance between different genotypes can be identified in leaf sections.

Cite this article

Ying Zhang, Yingxia Yang, Yanhong Jia, Xiangming Zhou, Lili Nie, Yue Zhang, Shouyi Chen, Jingan Wang, Zhongqi Liu . Using Leaf Sections to Identify Different Salt Tolerance Potential of Poplar Plants[J]. Chinese Bulletin of Botany, 2011 , 46(3) : 302 -310 . DOI: 10.3724/SP.J.1259.2011.00302

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