Chinese Bulletin of Botany >

2011 , Vol. 46 >Issue 3: 311 - 318

DOI: https://doi.org/10.3724/SP.J.1259.2011.00311

Expression of Keratinocyte Growth Factor-1 (KGF-1) in Transgenic Safflower

  • JIANG Ying ,
  • LIU Xiu-Meng ,
  • MA Ji-Qing ,
  • LI Wei ,
  • ZHU Hai-Lin ,
  • DU Mei-Li ,
  • LI Hai-Yan ,
  • LI Jiao-Kun
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  • 1Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development,Jilin Agricultural University, Changchun 130118, China;

    2College of Chinese Medicinal, Jilin Agricultural University,Changchun 130118, China;

    3College of Life Sciences, Jilin Agricultural University, Changchun 130118, China

Received date: 2010-12-01

  Revised date: 2011-01-24

  Online published: 2011-05-26

Supported by

National High Technology Research and Development Program of China (863 Program);the Science and Technology Development Projects of Jinlin Province;Important Projects in the Scientific Innovation of Colleges and Universities in China

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Abstract

The recombinant plasmid vector p139035S-KGF1 was constructed and transfected into safflower by Agrobacterium tumefaciens transfection. The period of infection to rooting was 14 weeks, and transformation efficiency was about 0.1%. Adventitious shoots were generated after cotyledons were cultured in selecting media containing hygromycin for 4–5 weeks. Rooted safflower was obtained in 4–8 weeks. KGF-1 was transformed into safflower chromosomes and expressed successfully, as determined by PCR, Southern blot, RT-PCR and Western blot analysis, which established the basis for the production of KGF-1.

Cite this article

JIANG Ying , LIU Xiu-Meng , MA Ji-Qing , LI Wei , ZHU Hai-Lin , DU Mei-Li , LI Hai-Yan , LI Jiao-Kun . Expression of Keratinocyte Growth Factor-1 (KGF-1) in Transgenic Safflower[J]. Chinese Bulletin of Botany, 2011 , 46(3) : 311 -318 . DOI: 10.3724/SP.J.1259.2011.00311

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