Chinese Bulletin of Botany >

2010 , Vol. 45 >Issue 04: 404 - 410

DOI: https://doi.org/10.11983/CBB29290

Genetic Mapping and Functional Analysis of Arabidopsis thaliana Male Sterile Mutant ms1142

  • Que Zhou ,
  • Zhongnan Yang ,
  • Sen Zhang ,
  • Yuhua Chang
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  • College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China

Received date: 2009-09-09

  Online published: 2010-09-26

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Abstract

A male sterile mutant, ms1142, was isolated from a population of ethyl methane sulfonate (EMS)-treated wild-type Arabidopsis thaliana. The mutant has small siliques with no seed set. Cytological observation showed that during anther development, the microspores ruptured and no pollen grains formed in the mutant anther locules. Scanning electron microscopy revealed an aberrant and broken microspore exine in the mutant. Genetic analysis indicated that the mutant is controlled by a single recessive nuclear gene. Map-based cloning mapped the mutant gene MS1142 to a region of 44 kb in BAC F16P17 on chromosome 1. Bioinformatics analysis revealed no previous male sterile gene cloned in this region, so MS1142 is a new gene involved in anther and pollen development. These results set the basis for cloning and functional analysis of this gene controlling anther development.

Key words： anther development; Arabidopsis thaliana

Cite this article

Que Zhou , Zhongnan Yang , Sen Zhang , Yuhua Chang . Genetic Mapping and Functional Analysis of Arabidopsis thaliana Male Sterile Mutant ms1142[J]. Chinese Bulletin of Botany, 2010 , 45(04) : 404 -410 . DOI: 10.11983/CBB29290

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