技术方法

An Improved Clearing and De-waxing Method for Plant Paraffin Sectioning

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  • College of Horticulture and Garden Architecture, Hainan University, Danzhou 571737, China

Received date: 2008-05-26

  Revised date: 2008-10-05

  Online published: 2009-03-01

Abstract

Paraffin sectioning plays an important role in plant science research. It is used widely in histology to observe and explain microstructure and provides a credible method for development of plant histology. This study aimed to investigate the limitations in traditional paraffin sectioning, such as the complex and time-consuming traditional protocol for improved measure. Xylene, a kind of neurotoxicant, is often used as a dewaxing and clearing chemical in the materials-preparation process. We substituted histolene (C1OH16), van-clear, oil of turpentine and n-butanol for xylene, to avoid a toxic effect. We improved the method to simplify the process of paraffin sectioning, shorten the cycle of the experiment and obtain quality slides.

Cite this article

Hubiao Yang;Xiaoxia Li;Lijuan Luo* . An Improved Clearing and De-waxing Method for Plant Paraffin Sectioning[J]. Chinese Bulletin of Botany, 2009 , 44(02) : 230 -235 . DOI: 10.3969/j.issn.1674-3466.2009.02.013

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