Embryo Germination and Rapid Propagation of Rare and Endangered Plant <EM>Davidia involucrata</EM>

Amei Yu;Zhixian Su*;Liqiang Wang;Zhirong Fang;Lijuan Zou

doi:10.3969/j.issn.1674-3466.2009.04.011

Chinese Bulletin of Botany >

2009 , Vol. 44 >Issue 04: 491 - 496

DOI: https://doi.org/10.3969/j.issn.1674-3466.2009.04.011

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Embryo Germination and Rapid Propagation of Rare and Endangered Plant Davidia involucrata

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1Key Laboratory of Environmental and Biodiversity Conservation, China West Normal University, Nanchong 637002, China2Mianyang Normal College, Mianyang 621000, China

Received date: 2008-07-28

Revised date: 2008-11-24

Online published: 2009-07-01

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Abstract

Three factors (media type, gibberellic acid [GA₃] and active carbon) affecting the embryo germination of Davidia involucrata were optimized by use of a uniform design and analyzed by regression analysis . Then a system for micropropagating D. involucrata was established from the embryos . From regression equations, the optimal conditions for embryo germination were the addition of 2.85 mg.L^-1 GA₃ and 0.25 g.L^-1 activated carbon to 1/2 MS medium containing 0.5 mg.L^-1 IBA and 1 mg.L^-1 6-BA. The germination rate of embryo was 87.72%, without significant deviation to the predicted value. The optimal conditions for shoot induction and multiplication were WPM medium supplemented with 0.1 mg.L^-1 NAA and 2.0 mg.L^-1 6-BA; the mean shoots obtained per explant was 4.34. An amount of 90% of shoots rooted on rooting medium, and 71% plants survived after transplanting.

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Amei Yu;Zhixian Su*;Liqiang Wang;Zhirong Fang;Lijuan Zou . Embryo Germination and Rapid Propagation of Rare and Endangered Plant Davidia involucrata[J]. Chinese Bulletin of Botany, 2009 , 44(04) : 491 -496 . DOI: 10.3969/j.issn.1674-3466.2009.04.011

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