In recent years, plant proteomics have advanced from a qualitative to an accurate quantitative stage. Original research articles published in 2007 and 2008 numbered nearly 160. Most of them reported on the proteomic features of plant tissue(organs) and organelles, plant development, plant response to environmental stress, and post-translational modification and protein-protein interactions. These proteome analyses mainly involved continuously improved 2-D electrophoresis-mass spectrometry (2DE-MS), multidimensional protein identification technology (MudPIT), and second-generation proteomics techniques such as 2-D fluorescence difference gel electrophoresis (2-D DIGE), ¹⁵N metabolic labeling, isotope-coded affinity tag (ICAT) and isobaric tags for relative and absolute protein quantitation (iTRAQ). This paper gives an overview of the research advances of plant proteomics in the past two years.