技术方法

Transformation of Populus euphratica

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  • 1National Engineering Laboratory for Tree Breeding, Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University; 2Agriculture and Animal Husbandry Bureau of Alashan League of Inner Mongolia, Bayanhot

Received date: 2009-03-06

  Revised date: 2009-09-03

  Online published: 2009-11-01

Abstract

The uniquearbor tree species Populus euphratica can grow well in arid desert. We established an Agrobacterium based transformation system for this species using a GUS gene as the reporter. Shoots of P. euphratica were regenerated from leaves placed on 1/2 MS supplemented with 1.0 mmol.L-1 6-BA and 5.0 mmol.L-1 NAA, leaves were infected for 15 min in a solution of A. tumefaciens with 0.4-0.6 OD600 and 100 mmol.L-1 AS, and then co-cultured for 2 days. A. tumefaciens was inhibited with 300 mg.L-1 Cef, and transgenic tissues and shoots were selected with 9 mg.L-1 G418.

Cite this article

Yuanwei Fan;Aizhi Liu;Huafang Wang . Transformation of Populus euphratica[J]. Chinese Bulletin of Botany, 2009 , 44(06) : 728 -734 . DOI: 10.3969/j.issn.1674-3466.2009.06.011

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