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技术方法

Anther Culture and Plant Regeneration of Dendrocalamus latiflorus

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  • 1Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, China
    2State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China

Received date: 2009-01-21

  Revised date: 2009-03-31

  Online published: 2010-01-01

Abstract

Embryogenic callus was initiated from bamboo (Dendrocalamus latiflorus Munro) anthers cultured on M8 medium supplemented with 2 mg·L–1 NAA, 0.5 mg·L–1 6-BA, 15 mg·L–1 phenylacetic acid (PAA), 7.5 mg·L–1 silver thiosulfate (STS), 500 mg·L–1 casein enzymatic hydrolysate (CH), 100 mg·L–1 proline, 100 mg·L–1 glutamine, 5.4% maltose, and 0.8% agar. Subculture of these embryogenic calli on the same medium resulted in embryoid formation and their subsequent germination to form rooted plantlets. A one-step method for anther culture and plant regeneration of D. latiflorus was preliminarily established.

Cite this article

Guirong Qiao;Haiying Li;Jing Jiang;Zongxiu Sun;Renying Zhuo* . Anther Culture and Plant Regeneration of Dendrocalamus latiflorus[J]. Chinese Bulletin of Botany, 2010 , 45(01) : 88 -90 . DOI: 10.3969/j.issn.1674-3466.2010.01.012

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