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Chinese Bulletin of Botany >

2010 , Vol. 45 >Issue 05: 579 - 587

DOI: https://doi.org/10.3969/j.issn.1674-3466.2010.05.007

Reference Genes for Real-time Fluorescence Quantitative PCR in Camellia sinensis

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  • 1Key Laboratory of Tea Biochemistry and Biotechnology, Ministry of Agriculture, Anhui Agricultural University, Hefei 230036,China

    2School of Biology Science, Anhui Agricultural University, Hefei 230036, China

Received date: 2009-12-08

  Revised date: 2010-03-30

  Online published: 2010-09-20

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Abstract

The selection of a suitable reference gene is an important prerequisite for successful gene expression analysis by real-time fluorescence quantitative PCR (qPCR). We investigated the expression stability of 4 endogenous candidate genes (18S rRNA, GAPDH, β-actin and α-tubulin) in qPCR experiments in different organs and tissues, including buds, leaves, young roots, stem, petals, seeds, and callus, of the tea plant Camellia sinensis (L.) O. Kuntze. The analysis with GeNorm and NormFinder algorithms revealed that β-actin could be used as a reference gene for organs and tissues and GAPDH for mature leaves and callus.

Cite this article

Meilian Sun, Yunsheng Wang, Dongqing Yang, Chaoling Wei, Liping Gao, Tao Xia, Yu Shan, Yang Luo . Reference Genes for Real-time Fluorescence Quantitative PCR in Camellia sinensis[J]. Chinese Bulletin of Botany, 2010 , 45(05) : 579 -587 . DOI: 10.3969/j.issn.1674-3466.2010.05.007

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