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DOI: https://doi.org/10.11983/CBB24156

RESEARCH ARTICLE

Cloning and Functional Analysis of 14-3-3 Protein gene TaGRF3-D in wheat (Triticum aestivum)

  • XUN Ru ,
  • GUO Shu-Juan ,
  • DIAO Hui-Xian ,
  • MA Meng ,
  • LIU Xiang-Li
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  • College of Life Science, Northwest A&F University, Yangling 712100, China

Received date: 2024-10-17

  Revised date: 2025-03-04

  Online published: 2025-03-18

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Abstract

INTRODUCTION: The 14-3-3 proteins are a highly conserved protein family that can specifically recognize phosphorylated target proteins playing a crucial role in plant abiotic stress responses. By interacting with AREBs/ABFs (ABA-responsive element binding proteins/ABA-responsive element binding factors) transcription factors, the 14-3-3 proteins can participate in ABA signal transduction and regulate abiotic stress tolerance. TaGRF3-D is a 14-3-3 protein in wheat (Triticum aestivum), our previous studies showed that the expression of this gene was up-regulated under ABA and abiotic stress.



RATIONALE: To explore the function of TaGRF3-D gene, the gene was cloned, and its subcellular localization and function on drought stress were investigated.



RESULTS: The results showed that the TaGRF3-D is highly conserved in monocotyledonous plants and is located in the nucleus and cell membrane. Compaired with wild type, the Arabidopsis thaliana transgenic lines overexpressed TaGRF3-D had significantly longer roots under PEG and ABA treatments, and demonstrated a significantly higher survival rate after drought stress. Further yeast two-hybrid analysis showed that the TaGRF3-D could interact with wheat TaABF3-B, TaABF4-A, TaABF15-D, TaABF16-B, TaABF17-D and TaABF18-B, but not with TaABF1-D, TaABF2-A and TaABF19-A.



CONCLUSION: These results indicate that TaABF3-D may respond to ABA signals by interacting with wheat TaABFs transcription factors, thereby enhancing drought stress tolerance of transgenic plants.




The phenotypes (A) and survival rates (B) of the TaGRF3-D transgenic lines and the wild type under drought stress



Key words： 14-3-3 protein; drought stress; ABA response; protein interaction

Cite this article

XUN Ru , GUO Shu-Juan , DIAO Hui-Xian , MA Meng , LIU Xiang-Li . Cloning and Functional Analysis of 14-3-3 Protein gene TaGRF3-D in wheat (Triticum aestivum)[J]. Chinese Bulletin of Botany, 0 : 1 -0 . DOI: 10.11983/CBB24156

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