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Chinese Bulletin of Botany >

2025 , Vol. 60 >Issue 4: 551 - 561

DOI: https://doi.org/10.11983/CBB24154

RESEARCH ARTICLES

Mechanism by which AtFTCD-L is Involved in the Root Response to Soil Compaction

  • Yuhan Liu ,
  • Qijiang Cao ,
  • Shihan Zhang ,
  • Yihui Li ,
  • Jing Wang ,
  • Xiaomeng Tan ,
  • Xiaoru Liu ,
  • Xianling Wang
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  • 1Key Laboratory of Urban Pest Control and Ecological Security in Liaoning, College of Life Sciences and Engineering, Shenyang University, Shenyang 110044, China
    2College of Biological Science and Technology, Shenyang Agricultural University, Shenyang 110866, China
*E-mail: caojiang2010@126.com

Received date: 2024-10-13

  Accepted date: 2025-01-20

  Online published: 2025-01-22

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Abstract

INTRODUCTION: Plant roots respond to various abiotic stresses, including drought stress, heavy metal stress, salt stress, and deficiencies in essential nutrients, during their growth and development. Among these factors, soil structure, especially soil compaction, significantly affects root growth and morphology, ultimately influencing crop yield.

RATIONALE: The Golgi apparatus plays a role in root growth and responds to abiotic stress through vesicle secretion. However, the mechanisms by which the Golgi apparatus contributes to the response of the root system to soil compaction remain unclear. Previous studies have demonstrated that AtFTCD-L in Arabidopsis is located on the trans-Golgi network (TGN) opposite the Golgi apparatus, and plays a role in vesicle sorting and/or secretion regulation of mucin components in the peripheral cells of the root cap.

RESULTS: Compared with those of the wild type, the root tips and root tip cells of the ftcd mutant are shorter in the longitudinal direction, but wider in the transverse direction, indicating abnormal cell morphology. Analysis of fluorescent signals from PIN-GFP plants revealed that PIN7 was either not expressed or expressed at very low levels in mutants. This study provides theoretical insights into the adaptive mechanisms of plant roots in response to abiotic stress induced by soil compaction.

CONCLUSION: In summary, AtFTCD-L responds to soil compaction in the roots of Arabidopsis by regulating the distribution or expression of PIN7.

Phenotypic differences in the effects of soil compaction on AtFTCD-L (WT)-, and mutant (ftcd)-mediated PIN7 regulation of root cell growth. The growth phenotypes of the root tips of the Arabidopsis lines on the 7th day.

Key words： AtFTCD-L; compaction; root tip; Arabidopsis thaliana; PIN7

Cite this article

Yuhan Liu , Qijiang Cao , Shihan Zhang , Yihui Li , Jing Wang , Xiaomeng Tan , Xiaoru Liu , Xianling Wang . Mechanism by which AtFTCD-L is Involved in the Root Response to Soil Compaction[J]. Chinese Bulletin of Botany, 2025 , 60(4) : 551 -561 . DOI: 10.11983/CBB24154

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References

[1] Adamowski M, Friml J (2015). PIN-dependent auxin transport: action, regulation, and evolution. Plant Cell 27, 20-32.
[2] Akula NN, Abdelhakim L, Knazovicky M, Ottosen CO, Rosenqvist E (2024). Plant responses to co-occurring heat and water deficit stress: a comparative study of tolerance mechanisms in old and modern wheat genotypes. Plant Physiol Biochem 210, 108595.
[3] Bakker PAHM, Pieterse CMJ, de Jonge R, Berendsen RL (2018). The soil-borne legacy. Cell 172, 1178-1180.
[4] Baskin TI (2005). Anisotropic expansion of the plant cell wall. Annu Rev Cell Dev Biol 21, 203-222.
[5] Bengough AG, McKenzie BM, Hallett PD, Valentine TA (2011). Root elongation, water stress, and mechanical impedance: a review of limiting stresses and beneficial root tip traits. J Exp Bot 62, 59-68.
[6] Blilou I, Xu J, Wildwater M, Willemsen V, Paponov I, Friml J, Heidstra R, Aida M, Palme K, Scheres B (2005). The PIN auxin efflux facilitator network controls growth and patterning in Arabidopsis roots. Nature 433, 39-44.
[7] Cao QJ, Zhang W, Liu XY, Li Y (2022). AtFTCD-L, a trans-Golgi network localized protein, modulates root grow- th of Arabidopsis in high-concentration agar culture medium. Planta 256, 3.
[8] Clark LJ, Whalley WR, Barraclough PB (2001). Partial mechanical impedance can increase the turgor of seedling pea roots. J Exp Bot 52, 167-171.
[9] Dai YJ, Luo XF, Zhou WG, Chen F, Shuai HW, Yang WY, Shu K (2019). Plant systemic signaling under biotic and abiotic stresses conditions. Chin Bull Bot 54, 255-264. (in Chinese)
  代宇佳, 罗晓峰, 周文冠, 陈锋, 帅海威, 杨文钰, 舒凯 (2019). 生物和非生物逆境胁迫下的植物系统信号. 植物学报 54, 255-264.
[10] Dinneny JR, Long TA, Wang JY, Jung JW, Mace D, Pointer S, Barron C, Brady SM, Schiefelbein J, Benfey PN (2008). Cell identity mediates the response of Arabidopsis roots to abiotic stress. Science 320, 942-945.
[11] Drakakaki G, Robert S, Szatmari AM, Brown MQ, Nagawa S, Van Damme D, Leonard M, Yang ZB, Girke T, Schmid SL, Russinova E, Friml J, Raikhel NV, Hicks GR (2011). Clusters of bioactive compounds target dynamic endomembrane networks in vivo. Proc Natl Acad Sci USA 108, 17850-17855.
[12] Gendre D, Oh J, Boutté Y, Best JG, Samuels L, Nilsson R, Uemura T, Marchant A, Bennett MJ, Grebe M, Bhalerao RP (2011). Conserved Arabidopsis ECHIDNA protein mediates trans-Golgi-network trafficking and cell elongation. Proc Natl Acad Sci USA 108, 8048-8053.
[13] Gerttula S, Zinkgraf M, Muday GK, Lewis DR, Ibatullin FM, Brumer H, Hart F, Mansfield SD, Filkov V, Groover A (2015). Transcriptional and hormonal regulation of gravi- tropism of woody stems in Populus. Plant Cell 27, 2800-2813.
[14] Hawes M, Allen C, Turgeon BG, Curlango-Rivera G, Minh Tran T, Huskey DA, Xiong ZG (2016). Root border cells and their role in plant defense. Annu Rev Phytopathol 54, 143-161.
[15] Hu J, Su HL, Cao H, Wei HB, Fu XK, Jiang XM, Song Q, He XH, Xu CZ, Luo KM (2022). AUXIN RESPONSE FACTOR7 integrates gibberellin and auxin signaling via interactions between DELLA and AUX/IAA proteins to regulate cambial activity in poplar. Plant Cell 34, 2688-2707.
[16] Jin K, Shen JB, Ashton RW, Dodd IC, Parry MAJ, Whalley WR (2013). How do roots elongate in a structured soil? J Exp Bot 64, 4761-4777.
[17] Kolb E, Legué V, Bogeat-Triboulot MB (2017). Physical root-soil interactions. Phys Biol 14, 065004.
[18] Livak KJ, Schmittgen TD (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods 25, 402-408.
[19] Logemann E, Birkenbihl RP, ülker B, Somssich IE (2006). An improved method for preparing Agrobacterium cells that simplifies the Arabidopsis transformation protocol. Plant Methods 2, 16.
[20] Luo J, Zhou JJ, Zhang JZ (2018). Aux/IAA gene family in plants: molecular structure, regulation, and function. Int J Mol Sci 19, 259.
[21] Lynch JP, Brown KM (2012). New roots for agriculture: exploiting the root phenome. Philos Trans Roy Soc B: Biol Sci 367, 1598-1604.
[22] Ma YR, Xu JH, Qi JH, Zhao D, Jin M, Wang T, Yang YF, Shi HJ, Guo L, Zhang H (2024). Crosstalk among plant hormone regulates the root development. Plant Signal Be- hav 19, 2404807.
[23] Marhava P, Bassukas AEL, Zourelidou M, Kolb M, Moret B, Fastner A, Schulze WX, Cattaneo P, Hammes UZ, Schwechheimer C, Hardtke CS (2018). A molecular rheostat adjusts auxin flux to promote root protophloem differentiation. Nature 558, 297-300.
[24] Okamoto T, Tsurumi S, Shibasaki K, Obana Y, Takaji H, Oono Y, Rahman A (2008). Genetic dissection of hormonal responses in the roots of Arabidopsis grown under continuous mechanical impedance. Plant Physiol 146, 1651-1662.
[25] Pan JW, Ye D, Wang LL, Hua J, Zhao GF, Pan WH, Han N, Zhu MY (2004). Root border cell development is a temperature-insensitive and Al-sensitive process in barley. Plant Cell Physiol 45, 751-760.
[26] Petricka JJ, Winter CM, Benfey PN (2012). Control of Arabidopsis root development. Annu Rev Plant Biol 63, 563-590.
[27] Reiter WD (2008). Biochemical genetics of nucleotide sugar interconversion reactions. Curr Opin Plant Biol 11, 236-243.
[28] Sampedro J, Gianzo C, Iglesias N, Guitián E, Revilla G, Zarra I (2012). AtBGAL10 is the main xyloglucan β-galactosidase in Arabidopsis, and its absence results in unusual xyloglucan subunits and growth defects. Plant Physiol 158, 1146-1157.
[29] Shekhar V, St?ckle D, Thellmann M, Vermeer JEM (2019). The role of plant root systems in evolutionary adaptation. Curr Top Dev Biol 131, 55-80.
[30] Su NN, Zhu AQ, Tao X, Ding ZJ, Chang SH, Ye F, Zhang Y, Zhao C, Chen Q, Wang JQ, Zhou CY, Guo YR, Jiao SS, Zhang SF, Wen H, Ma LX, Ye S, Zheng SJ, Yang F, Wu S, Guo JT (2022). Structures and mechanisms of the Arabidopsis auxin transporter PIN3. Nature 609, 616-621.
[31] Uchida K, Blumstein DT, Soga M (2024). Managing wildlife tolerance to humans for ecosystem goods and services. Trends Ecol Evol 39, 248-257.
[32] Waadt R, Seller CA, Hsu PK, Takahashi Y, Munemasa S, Schroeder JI (2022). Plant hormone regulation of abiotic stress responses. Nat Rev Mol Cell Biol 23, 680-694.
[33] Wang PF, Chen XS, Goldbeck C, Chung E, Kang BH (2017). A distinct class of vesicles derived from the trans- Golgi mediates secretion of xylogalacturonan in the root border cell. Plant J 92, 596-610.
[34] Whitmore AP, Whalley WR (2009). Physical effects of soil drying on roots and crop growth. J Exp Bot 60, 2845-2857.
[35] Williams A, de Vries FT (2020). Plant root exudation under drought: implications for ecosystem functioning. New Phytol 225, 1899-1905.
[36] Xiong YW, Li XW, Wang TT, Gong Y, Zhang CM, Xing K, Qin S (2020). Root exudates-driven rhizosphere recruitment of the plant growth-promoting rhizobacterium Bacillus flexus KLBMP 4941 and its growth-promoting effect on the coastal halophyte Limonium sinense under salt stress. Ecotoxicol Environ Saf 194, 110374.
[37] Xu ED, Wu MY, Liu YY, Tai YP, Zha WJ, Gong CY, Zou Y, Zhang PJ, Zhang W, Chen X (2023). The Golgi-localized transporter OsPML3 is involved in manganese homeostasis and complex N-glycan synthesis in rice. J Exp Bot 74, 1853-1872.
[38] Yan X, Wang YT, Xu M, Dahhan DA, Liu C, Zhang Y, Lin JX, Bednarek SY, Pan JW (2021). Cross-talk between clathrin-dependent post-Golgi trafficking and clathrin-mediated endocytosis in Arabidopsis root cells. Plant Cell 33, 3057-3075.
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