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Chinese Bulletin of Botany >

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DOI: https://doi.org/10.11983/CBB24113

TECHNIQUES AND METHODS

A Preliminary Study on Tissue Culture and Rapid Propagation Technology of Phyllanthus acidus

  • LI Tong ,
  • LI Chu-Ran ,
  • ZHANG Zhi-Yu ,
  • FU Xiao-Man ,
  • LIU Yun ,
  • YANG Xiao-Qin ,
  • ZHANG Ying-Jun ,
  • YANG Li-Ying ,
  • DIAO Beng
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  • 1Key Laboratory of State Forestry and Grassland Administration on Highly-Efficient Utilization of Forestry Biomass Resources in Southwest China, Southwest Forestry University, Kunming 650224, China; 2Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; 3Collaborative Yunnan Xinxing Greening Engineering Co., Ltd., Kunming 650228, China

Received date: 2024-07-23

  Revised date: 2024-10-29

  Online published: 2024-12-27

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Abstract

Phyllanthus acidus (L.) Skeels is a tropical tree with both edible and medicinal properties. Exploring its tissue culture and rapid propagation technology can promote the sustainable development and utilization of its resources. In this study, the stem tips of P. acidus were used as explants, and the schemes of primary culture, secondary proliferation culture and rooting culture were screened. The results showed that the optimal medium for primary culture was MS+2.0 mg·L–1 6-BA+0.2 mg·L–1 NAA, and the induced germination rate of explants reached 81.11%. The optimal medium for subculture was MS+1.0 mg·L–1 6-BA+0.2 mg·L–1 IBA, and the proliferation coefficient was 1.86. The optimal medium for rooting was MS+1.5 mg·L–1 IBA, and the rooting rate reached 83.00%. After 7 days of cultivation, the seedlings were transplanted with perlite, peat soil and humus=1:1:1 (v/v/v), and the survival rate was 90%. This study took about 90 days from inoculation of explants to obtaining regenerated plants, providing technical support for the resource conservation and industrial application of P. acious.

Key words： Phyllanthus acidus; tissue culture; rapid propagation

Cite this article

LI Tong , LI Chu-Ran , ZHANG Zhi-Yu , FU Xiao-Man , LIU Yun , YANG Xiao-Qin , ZHANG Ying-Jun , YANG Li-Ying , DIAO Beng .

A Preliminary Study on Tissue Culture and Rapid Propagation Technology of Phyllanthus acidus[J]. Chinese Bulletin of Botany, 0 : 1 -0 . DOI: 10.11983/CBB24113

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