A Preliminary Study on Tissue Culture and Rapid Propagation Technology of Phyllanthus acidus
- 1Key Laboratory of State Forestry and Grassland Administration on Highly-Efficient Utilization of Forestry Biomass Resources in Southwest China, Southwest Forestry University, Kunming 650224, China
2Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
3Yunnan Xinxing Greening Engineering Co., Ltd., Kunming 650228, China
Received date: 2024-07-23
Accepted date: 2024-12-26
Online published: 2024-12-27
Abstract
INTRODUCTION A preliminary tissue culture system for Phyllanthus acidus was established.
RATIONALE In this study, the stem tips of P. acidus were used as explants, and the schemes of primary culture, secondary proliferation culture and rooting culture were screened.
RESULTS The results showed that the optimal medium for primary culture was MS+2.0 mg·L-1 6-BA+0.2 mg·L-1 NAA, and the induced germination rate of explants reached 81.11%. The optimal medium for subculture was MS+1.0 mg·L-1 6-BA+0.2 mg·L-1 IBA, and the proliferation coefficient was 1.86. The optimal medium for rooting was MS+1.5 mg·L-1 IBA, and the rooting rate reached 83.00%. After 7 days of cultivation, the plantlets were transplanted with perlite, peat soil and humus=1:1:1 (v/v/v), and the survival rate was 90%.
CONCLUSION The most suitable medium for primary culture using the stem tip of P. acidus as explant was MS medium and the phytohormones were 2.0 mg·L-1 6-BA and 0.2 mg·L-1 NAA; the optimal phytohormones for subculture were 1.0 mg·L-1 6-BA and 0.2 mg·L-1 IBA; the optimal phytohormone for rooting culture was 1.5 mg·L-1 IBA.
Key words： Phyllanthus acidus; tissue culture; rapid propagation
Cite this article
Tong Li , Churan Li , Zhiyu Zhang , Xiaoman Fu , Yun Liu , Yingjun Zhang , Liying Yang , Ping Zhao . A Preliminary Study on Tissue Culture and Rapid Propagation Technology of Phyllanthus acidus[J]. Chinese Bulletin of Botany, 2025 , 60(4) : 611 -620 . DOI: 10.11983/CBB24113
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