Chinese Bulletin of Botany ›› 2023, Vol. 58 ›› Issue (4): 560-572.DOI: 10.11983/CBB22123
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Changjie Chen, Yuhuan Miao, Dandan Luo, Zixin Wang, Lujuan Guo, Tingting Zhao(), Dahui Liu(
)
Received:
2022-06-17
Accepted:
2022-11-15
Online:
2023-07-01
Published:
2022-11-15
Contact:
*E-mail: datingsdau@126.com;liudahui@hbtcm.edu.cn
Changjie Chen, Yuhuan Miao, Dandan Luo, Zixin Wang, Lujuan Guo, Tingting Zhao, Dahui Liu. Cloning and Functional Verification of the Borneol Dehydrogenase Encoding Gene AArBDH1 in Artemisia argyi[J]. Chinese Bulletin of Botany, 2023, 58(4): 560-572.
Figure 2 Relative contents of borneol and camphor in the leaves of 35 Artemisia argyi varieties S01-S35 is the number of 35 Artemisia argyi varieties.
No. | Gene | Species | GenBank accession | Functional annotation | Reference |
---|---|---|---|---|---|
1 | AaBDH | Artemisia annua | KT070864 | Borneol dehydrogenase | Tian et al., |
2 | ADH2 | A. annua | GU253890 | Alcohol dehydrogenase | Polichuk et al., |
3 | ALDH | Pseudomonas aeruginosa | WP_028626884 | Dehydrogenase | Tsang et al., |
4 | CcBDH1 | Cinnamomum camphora | MW012431 | Borneol dehydrogenase | Ma et al., |
5 | CcBDH2 | C. camphora | MW012432 | Borneol dehydrogenase | Ma et al., |
6 | CcBDH3 | C. camphora | MN551096 | Borneol dehydrogenase | Ma et al., |
7 | CHCR | Cricetulus griseus | AB020238 | Carbonyl reductase | Terada et al., |
8 | DlSDR | Digitalis lanata | Q93Y47 | 3-beta-hydroxysteroid dehydrogenase | Finsterbusch et al., |
9 | FiSDR | Forsythia intermedia | AF352735 | Secoisolariciresinol dehydrogenase | Xia et al., |
10 | ISPD | Mentha × piperita | AAU20370 | (-)-isopiperitenol dehydrogenase | Ringer et al., |
11 | HSCR | Homo sapiens | J04056 | Carbonyl reductase | Wermuths et al., |
12 | LiBDH | Lavandula × intermedia | AFV30207 | Borneol dehydrogenase | Sarker et al., |
13 | MIR | Mentha × piperita | AY300162 | (-)-isopiperitenone reductase | Ringer et al., |
14 | MNR | Mentha × piperita | ABC88670 | (+)-neomenthol reductase | Davis et al., |
15 | MMR | Mentha × piperita | AAQ55960 | Menthol dehydrogenase | Davis et al., |
16 | NEPS | Nepeta mussinii | 6F9Q_D | Nepetalactol-related short-chain dehydrogenase | Lichman et al., |
17 | PsSDR | Pisum sativum | AAF04253 | Short-chain alcohol dehydrogenase | Brosché and Strid, |
18 | PTCR | Sus scrofa | M80709 | 20-beta-hydroxysteroid dehydrogenase | Tanaka et al., |
19 | RED1 | A. annua | GU167953 | Reductase/dehydrogenase | Rydén et al., |
20 | SalR | Papaver bracteatum | EF184229 | Salutaridine reductase | Geissle et al., |
21 | ZSD1 | Zingiber zerumbet | AB480831 | Short-chain dehydrogenase | Okamoto et al., |
Table 1 Information of SDR encoding genes downloaded from NCBI
No. | Gene | Species | GenBank accession | Functional annotation | Reference |
---|---|---|---|---|---|
1 | AaBDH | Artemisia annua | KT070864 | Borneol dehydrogenase | Tian et al., |
2 | ADH2 | A. annua | GU253890 | Alcohol dehydrogenase | Polichuk et al., |
3 | ALDH | Pseudomonas aeruginosa | WP_028626884 | Dehydrogenase | Tsang et al., |
4 | CcBDH1 | Cinnamomum camphora | MW012431 | Borneol dehydrogenase | Ma et al., |
5 | CcBDH2 | C. camphora | MW012432 | Borneol dehydrogenase | Ma et al., |
6 | CcBDH3 | C. camphora | MN551096 | Borneol dehydrogenase | Ma et al., |
7 | CHCR | Cricetulus griseus | AB020238 | Carbonyl reductase | Terada et al., |
8 | DlSDR | Digitalis lanata | Q93Y47 | 3-beta-hydroxysteroid dehydrogenase | Finsterbusch et al., |
9 | FiSDR | Forsythia intermedia | AF352735 | Secoisolariciresinol dehydrogenase | Xia et al., |
10 | ISPD | Mentha × piperita | AAU20370 | (-)-isopiperitenol dehydrogenase | Ringer et al., |
11 | HSCR | Homo sapiens | J04056 | Carbonyl reductase | Wermuths et al., |
12 | LiBDH | Lavandula × intermedia | AFV30207 | Borneol dehydrogenase | Sarker et al., |
13 | MIR | Mentha × piperita | AY300162 | (-)-isopiperitenone reductase | Ringer et al., |
14 | MNR | Mentha × piperita | ABC88670 | (+)-neomenthol reductase | Davis et al., |
15 | MMR | Mentha × piperita | AAQ55960 | Menthol dehydrogenase | Davis et al., |
16 | NEPS | Nepeta mussinii | 6F9Q_D | Nepetalactol-related short-chain dehydrogenase | Lichman et al., |
17 | PsSDR | Pisum sativum | AAF04253 | Short-chain alcohol dehydrogenase | Brosché and Strid, |
18 | PTCR | Sus scrofa | M80709 | 20-beta-hydroxysteroid dehydrogenase | Tanaka et al., |
19 | RED1 | A. annua | GU167953 | Reductase/dehydrogenase | Rydén et al., |
20 | SalR | Papaver bracteatum | EF184229 | Salutaridine reductase | Geissle et al., |
21 | ZSD1 | Zingiber zerumbet | AB480831 | Short-chain dehydrogenase | Okamoto et al., |
Figure 5 Gene structure (A), expression profiles in different organizations of AArBDH1 (B) and protein structure analysis of AArBDH1 (C) UTR: Untranslated region; CDS: Coding sequence. Leaf A, Leaf B, Leaf C and Leaf D represent leaf buds, 15 days, 30 days and 45 days old leaves, respectively. Different lowercase letters indicate significant differences (P<0.05).
Figure 6 Protein structure prediction of AArBDH1 (A) The subcellular localization prediction of AArBDH1; (B) The secondary structure prediction of AArBDH1; (C) The tertiary structure prediction of AArBDH1
Figure 8 Induced expression product and purification of AA- rBDH1 1: Expression products without IPTG; 2: Induced expression by IPTG; 3: Primary purified protein; 4: Secondary purified protein
Figure 10 GC-MS analysis of products catalyzed by AArBDH1 CG: Control group; E1, E2: Experimental groups; A: Chromatographic peak; B: Fragment ion peak
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