Chinese Bulletin of Botany ›› 2007, Vol. 24 ›› Issue (05): 624-628.

• 技术与方法 • Previous Articles     Next Articles

An Effective Method for Extracting Total RNA from Young Ears of Maize

Yun Zhu Meng Wang Zhiwei Jia Yun Lian Ying Jin Guoying Wang   

  1. State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, China
  • Received:2006-11-02 Revised:2006-11-27 Online:2007-09-01 Published:2007-09-01
  • Contact: Guoying Wang

Abstract: Here we report a modified hot phenol method for extracting the total RNA from young ears of maize. This method involves wiping off polysaccharides by adding KAC in the extraction buffer. RNA isolated by this method is of high quality and can be used for the later molecular manipulations.