Chinese Bulletin of Botany ›› 2012, Vol. 47 ›› Issue (2): 149-154.DOI: 10.3724/SP.J.1259.2012.00149

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Metabolites Research of Corydalis yanhusuo Tubers with Gas Chromatography-mass Spectrometry

Pengfei Wang1, Qianqian Wang3, Xianen Li2*, Minjian Qin1*   

  1. 1China Pharmaceutical University, Nanjing 210009, China;

    2Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China;

    3Jilin Agricultural University, Changchun 130118, China
  • Received:2011-08-26 Revised:2011-11-30 Online:2012-03-01 Published:2012-03-16
  • Contact: Xianen Li,Minjian Qin

Abstract: We established the metabolomics method for investigating metabolites in Corydalis yanhusuo bulbs by gas chromatography-mass spectrometry. We also investigated the effect of different volumes of extracts and derived reagents (MSTFA) for peak signal intensity and numbers. We determined the best conditions with each 100 mg material extracted by use of 300 μL extract and 100 μL MSTFA. To detect samples of C. yanhusuo tuber, we obtained 92 kinds of compounds, which contained 5 fatty acids, 21 organic acids, 16 amino acids, 6 sugars, 23 other ingredients, and 21 unknown compounds. We also studied the pattern recognition of C. yanhusuo tuber metabolites by principal component analysis. We could provide valid data and reference values for further research into C. yanhusuo tubers.