Chinese Bulletin of Botany ›› 2008, Vol. 25 ›› Issue (06): 695-700.
• 实验简报 • Previous Articles Next Articles
Zhigang Huang, Huang Hu, Xuanzhi Zhao, Ruozhong Wang, Langtao Xiao *
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Abstract: The auxin receptor TIR1, which can directly bind to auxin through the formation of the SCFTIR1 complex , is the key protein in the Aux /IAA degradation pathway of the 26S proteasome. In this research, we designed a specific primer according to the results of a BLAST search of bioinformatics data; the super hybrid rice parental line 1S was used as the plant material. After RT-PCR and T-A cloning, a 2 219 bp cDNA sequence was obtained through sequencing. Its open reading frame (ORF) was 1 764 bp long and coded for a polypeptide of 587 amino acids. Bioinformatics analysis revealed that the polypeptide shares 77% similarity with AtTIR1. Both polypeptides contain an F-box motif and a leucine-rich repeat domain but no transmembrane helices or signal peptide. The cDNA sequence was designated as OsTIR1 and submitted to GenBank.
Zhigang Huang;Huang Hu;Xuanzhi Zhao;Ruozhong Wang;Langtao Xiao *. Cloning and Bioinformatics Analysis of TIR1 cDNA from Super Hybrid Rice Parental Line 1S[J]. Chinese Bulletin of Botany, 2008, 25(06): 695-700.
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https://www.chinbullbotany.com/EN/Y2008/V25/I06/695