Abstract: Conifers are high in polysaccharides and other secondary metabolites such as polyphenols, terpens and resins, which makes it rather difficult to obtain high quality genomic DNA from their tissues. Five methods,i.e. basic method, high salt precipitation, CTAB precipitation, Ziegenhagen's method and DNeasy Plant Mini Kits(QIAGEN), were used to extract genomic DNA from the needle leaves of [WTBX]Pinus massoniana.[WT] The resulted DNA samples of the five methods were tested using agarose gel electrophoresis, restriction digests and PCR. The difference in yield, quality, time duration and expense of the obtained DNA are compared, and CTAB precipitation was decided as the best methed for the practical work of molecular ecology.