Chinese Bulletin of Botany ›› 1999, Vol. 16 ›› Issue (04): 433-438.

• 研究报告 • Previous Articles     Next Articles

Effects of Perianth Explant Ages on Differentiation of Floral Organ in Hyacinthus orientalis L.

LI Xing-Guo and LU Wen-Liang   

  1. 1(Department of Plant Science, Shandong Agricultural University, Taian 271018) 2(Institute of Botany, The Chinese Academy of Sciences, Beijing 100093)
  • Received:1998-04-16 Revised:1998-06-02 Online:1999-07-20 Published:1999-07-20
  • Contact: LI Xing-Guo

Abstract: The effects of the perianth explants of different ages on direct regeneration of floral organs have been observed in perianth culture of Hyacinthus. The experiments revealed that: 1. When cultured on Murashige and Skoog (MS) medium supplemented with 6-BA 2 mg/L and 2,4-D 0.1 mg/L, plenty of explants at age V were senescent and lost their capacity for regenerating floral organs. However, many explants at ages Ⅱ-Ⅳ showed vitrification. 2. The explants which showed vitrification could regerminate normal tepals when transfered to the medium supplemented with 6-BA 0.2 mg/L and NAA 0.005 mg/L, which demonstrates that decreasing the concentration of exogenous hormones could inhibit the vitrification. 3. The early structure of the pistil could be induced to form from the morphologically lower past of the perianth cultured on MS supplemented with 6-BA 2 mg/L and 2,4-D 0.1 mg/L. The explants at age Ⅲ were advantageous for the inducement of such early structures.