Chinese Bulletin of Botany ›› 1999, Vol. 16 ›› Issue (04): 433-438.
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LI Xing-Guo and LU Wen-Liang
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Abstract: The effects of the perianth explants of different ages on direct regeneration of floral organs have been observed in perianth culture of Hyacinthus. The experiments revealed that: 1. When cultured on Murashige and Skoog (MS) medium supplemented with 6-BA 2 mg/L and 2,4-D 0.1 mg/L, plenty of explants at age V were senescent and lost their capacity for regenerating floral organs. However, many explants at ages Ⅱ-Ⅳ showed vitrification. 2. The explants which showed vitrification could regerminate normal tepals when transfered to the medium supplemented with 6-BA 0.2 mg/L and NAA 0.005 mg/L, which demonstrates that decreasing the concentration of exogenous hormones could inhibit the vitrification. 3. The early structure of the pistil could be induced to form from the morphologically lower past of the perianth cultured on MS supplemented with 6-BA 2 mg/L and 2,4-D 0.1 mg/L. The explants at age Ⅲ were advantageous for the inducement of such early structures.
LI Xing-Guo and LU Wen-Liang. Effects of Perianth Explant Ages on Differentiation of Floral Organ in Hyacinthus orientalis L.[J]. Chinese Bulletin of Botany, 1999, 16(04): 433-438.
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