Abstract: Using one-step Isothiocyanate method and RNeasy Plant Mini Kit, total RNA with high quality was extracted from Gerbera hybrida corolla tissue that is rich in polysaccharide. In order to remove polysaccharide interference in the procedure of RNA separation, we improved the method by adding denaturing_solution to dissolve the R NA precipitation and by prolonging the centrifugation time and dissolving time using DEPC H2O. These provided a convenient and effective method for research on gene expression in anthocyanin biosynthesis using Northern hybridization and change in flower color. Finally, different methods for RNA separation aer compared.