Chinese Bulletin of Botany ›› 2001, Vol. 18 ›› Issue (02): 226-230.
• 研究论文 •
ZHAO Yu-Jin WANG Tai
The change of α_amylase activity and isoenzyme in embryos of Nongk
en 58F, Nongken 58S and Nongken 58 were analyzed by assay of reducing sugars with 3,5_dinitrosalicylic acid(DNS) and acrylamide gel electrophoresis technique under the different germinating time. The α_amylase activity in the embryos of both Nongken 58F and Nongken 58S were higher than that of Nongken 58 from 0 to 36 h germinating time. Rice seed embryos had 5 isoenzyme bands(A1,A2,A3,A4,A5). A2,A4 and A5 were present in the embryos of Nongken 58F, Nongken 58S and Nongke
n 58. A3 was specific for both Nongken 58F and Nongken 58S. A1 was specific for the embryos of Nongken 58. These results suggested a higher α_amylase activity be necessary for promoting fast germination of rice seeds, α-amylase isoenzyme A1 and A3 may play an important role in regulation of germination speed.
ZHAO Yu-Jin WANG Tai. Analysis of the Relationship between α-Amylase and Germinating Rate of Rice Seeds during the Process of Seed Germination[J]. Chinese Bulletin of Botany, 2001, 18(02): 226-230.
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