Abstract: The guaiccal oxidase activity of Corilus versicolor was studied when it was cultured in PDY fluid medium. The peak of the enzyme production appeared in the 16th days under the castant temperature of 30 ℃ and vibration(110 r/min), the maxmum enzyme activity was 218.0 u. The optimum temperature was 30 ℃ and the optimum pH was 4.0, Mn2+,Ba2+,Mg2+,Cu2+ were activators for guaiccal oxidase, whereas Ag+ obviously inhibited the enzyme activity.