Abstract: In the report, the gramineous expression vectors pGU4AGBar and pGBIU4AGBar were used, respectively. The sgna gene, a synthetical agglutinin gene of Galanthus navies, had been transferred into the winter wheat varieties Xinong 2208 and Xinong 132 by pollen-tube pathway. The PCR and Southern blotting analysis showed that 20 transgenic plants with sgna gene were obtained. Western blot ting analysis revealed that the target protein was expressed in the transgenic plants. The transformation frequency were 0.28%～0.84%.