Chinese Bulletin of Botany ›› 2006, Vol. 23 ›› Issue (4): 389-394.
• 技术与方法 • Previous Articles Next Articles
Xinming Jing†, Guangkun Yin
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Abstract: A procedure for isolating high pure mitochondria from soybean (Glycine max) seeds by discontinuous Percoll density gradient centrifugation was established. The preparation contains more than 97% mitochondria as intact organelles with no detectable cytosol, peroxisomes and chlorophyll contamination. The mitochondria are stable at 0-4℃ for at least 2 hours.
Xinming JingGuangkun Yin. Isolation of High Pure Mitochondria from Soybean Seeds[J]. Chinese Bulletin of Botany, 2006, 23(4): 389-394.
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