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植物学报 >

2024 , Vol. 59 >Issue 2: 217 - 230

DOI: https://doi.org/10.11983/CBB23161

研究报告

水稻穗部性状QTL定位及候选基因分析

  • 朱超宇 ,
  • 胡程翔 ,
  • 朱哲楠 ,
  • 张芷宁 ,
  • 汪理海 ,
  • 陈钧 ,
  • 李三峰 ,
  • 连锦瑾 ,
  • 唐璐瑶 ,
  • 钟芊芊 ,
  • 殷文晶 ,
  • 王跃星 ,
  • 饶玉春
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  • 1浙江师范大学生命科学学院, 金华 321004
    2中国水稻研究所水稻生物育种全国重点实验室, 杭州 310006
* E-mail: wangyuexing@caas.cn;


饶玉春, 博士/博士后, 教授, 博士生导师, “双龙学者”特聘教授。浙江省高校领军人才培养计划高层次拔尖人才(2022年), 金华市青年拔尖人才(2023年), 浙江省发明协会理事、植物遗传专业委员会副主任, 中国作物学会会员, 浙江省遗传学会会员。主要从事水稻等禾谷类作物的分子遗传学研究, 以第一作者或者通讯作者身份在Science Bulletin、New Phytologist等国际著名学术期刊上发表论文80余篇, 以合作者身份在Nature Plants、Proc Natl Acad Sci USA等杂志上发表论文30余篇。主持转基因国家重大专项子课题、国家自然科学基金、浙江省自然科学基金等项目。授权国家发明专利25项(第一完成人)。E-mail: ryc@zjnu.cn

收稿日期: 2023-12-04

  录用日期: 2024-01-30

  网络出版日期: 2024-01-30

基金资助

浙江省自然科学基金(LZ23C130003);2024年国家级大学生创新创业训练计划和2024年浙江省大学生科技创新活动计划暨新苗人才计划

收起

Mapping of QTLs Associated with Rice Panicle Traits and Candidate Gene Analysis

  • Chaoyu Zhu ,
  • Chengxiang Hu ,
  • Zhenan Zhu ,
  • Zhining Zhang ,
  • Lihai Wang ,
  • Jun Chen ,
  • Sanfeng Li ,
  • Jinjin Lian ,
  • Luyao Tang ,
  • Qianqian Zhong ,
  • Wenjing Yin ,
  • Yuexing Wang ,
  • Yuchun Rao
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  • 1College of Life Sciences, Zhejiang Normal University, Jinhua 321004, China
    2State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China

Received date: 2023-12-04

  Accepted date: 2024-01-30

  Online published: 2024-01-30

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摘要

水稻(Oryza sativa)穗部性状与产量直接相关, 其相关基因的挖掘与功能解析对于保障国家粮食安全意义重大。以籼稻华占(HZ)和粳稻热研2号(Nekken2)及构建的120个重组自交系(RILs)为实验材料, 测定了穗长、每穗粒数、结实率、柱头外露率及一次枝梗数等穗部性状。结合高密度分子遗传图谱进行QTL定位, 结果共检测到31个QTLs, 分别位于第1、2、3、4、5、6、10和11号染色体上, 其中2个位点的LOD值分别高达5.45与5.28。通过分析筛选QTL区间内可能影响穗部性状的相关基因, 并利用qRT-PCR进行基因表达检测, 发现LOC_Os05g05490、LOC_Os05g06150、LOC_Os03g11700、LOC_Os03g12430、LOC_Os05g28720、LOC_Os05g30890、LOC_Os05g31740和LOC_Os02g17880在双亲间的表达水平差异显著。其中, 前5个基因编码三角状五肽重复蛋白, 而后3个基因编码糖基转移酶。研究挖掘到31个与穗部性状相关的QTLs, 为进一步定位和克隆相关基因, 从而选育高产水稻新品种奠定理论基础。

关键词： 水稻; 穗部性状; QTL定位; 候选基因; 高产

本文引用格式

朱超宇 , 胡程翔 , 朱哲楠 , 张芷宁 , 汪理海 , 陈钧 , 李三峰 , 连锦瑾 , 唐璐瑶 , 钟芊芊 , 殷文晶 , 王跃星 , 饶玉春 . 水稻穗部性状QTL定位及候选基因分析[J]. 植物学报, 2024 , 59(2) : 217 -230 . DOI: 10.11983/CBB23161

Abstract

Rice panicle traits have a distinct effect on yield, and the exploration and research of related genes play a crucial role in ensuring national food security. In this study, we used the indica rice HZ, the japonica rice Nekken2 and 120 recombinant inbred lines population constructed from them as experimental materials to measure panicle length, grain number per panicle, seed-setting rate, stigma exsertion rate, number of primary branches and other panicle traits. We combined the high-density genetic map for QTL mapping, a total of 31 QTLs were detected on chromosome 1, 2, 3, 4, 5, 6, 10 and 11, among which 2 LOD values were as high as 5.45 and 5.28. By analyzing the candidate genes in the QTL interval, the related genes that may affect the panicle traits were screened out, and qRT-PCR was used for gene expression analysis, which revealed that the expression levels of LOC_Os05g05490, LOC_Os05g06150, LOC_Os03g11700, LOC_Os03g12430, LOC_Os05g28720, LOC_Os05g30890, LOC_Os05g31740 and LOC_Os02g17880 were significantly different between the parents. Among these genes, the first five genes encode tripartite pentapeptide repeat proteins, while the latter three genes encode glycosyltransferases. This study identified 31 QTLs related to panicle traits, laying a theoretical basis for further localization and cloning of related genes and breeding new high-yield rice varieties.

Key words： Oryza sativa (rice); panicle traits; QTL mapping; candidate genes; high-yield

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