异源过表达齿肋赤藓ScABI3基因改变拟南芥气孔表型并提高抗旱性
收稿日期: 2020-12-26
录用日期: 2021-05-08
网络出版日期: 2021-05-08
基金资助
国家自然科学基金(31900270)
Heterologous Overexpression of Desiccation-tolerance Moss ScABI3 Gene Changes Stomatal Phenotype and Improves Drought Resistance in Transgenic Arabidopsis
Received date: 2020-12-26
Accepted date: 2021-05-08
Online published: 2021-05-08
ABI3是ABA信号通路中关键的转录调控因子, 参与种子休眠、质体发育及苔藓耐干等重要生理过程, 在植物抗逆中发挥关键作用。以荒漠耐干苔藓——齿肋赤藓(Syntrichia caninervis)为材料, 克隆了抗逆基因ScABI3并获得3个独立的pCAMBIA1301-ScABI3转基因拟南芥(Arabidopsis thaliana)纯合株系。结果表明, 转基因拟南芥叶片气孔孔径增大, 单位面积气孔数量减少, 植株水分利用效率提高; 在干旱处理14天后转基因拟南芥植株存活率显著高于野生型, 离体叶片失水率显著低于野生型。进一步研究发现, ScABI3转基因拟南芥通过提高自身活性氧(ROS)清除能力增强植株抗旱性。研究结果可为开发利用荒漠植物基因资源培育抗逆作物品种奠定基础。
张一弓, 张怡, 阿依白合热木·木台力甫, 张道远 . 异源过表达齿肋赤藓ScABI3基因改变拟南芥气孔表型并提高抗旱性[J]. 植物学报, 2021 , 56(4) : 414 -421 . DOI: 10.11983/CBB20212
ABI3 is a key transcriptional factor of the ABA signaling pathway, which is involved in seed dormancy, plastid development, and desiccation tolerance of bryophytes, and plays an important role in the stress tolerance of plant. This study obtained three independent pCAMBIA1301-ScABI3 transgenic Arabidopsis homozygous lines. The results showed that transgenic plants increased leaf stomata diameter, reduced the number of stomata in unit area, and improved plant water use efficiency; transgenic plants survival rate was significantly higher than wild type (WT) after 14 days of drought treatment; the water loss rate of transgenic leaves was significantly lower than that of WT. Further research has found that ScABI3 transgenic plants had higher drought resistance characteristics by improving reactive oxygen species (ROS) scavenging ability. These results may be contributed to the development and utilization of genetic resources of desert plants, also provide the theoretical and practical base for molecular breeding.
Key words： abscisic acid; ABI3; drought resistance; stomata
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