植物小RNA荧光原位杂交实验方法
收稿日期: 2020-04-02
录用日期: 2021-05-07
网络出版日期: 2021-05-07
基金资助
中国科学院战略性先导科技专项(A类) No(XDA24010106-2);国家自然科学基金No(31900391)
Protocols for Small RNA FISH in Plants
Received date: 2020-04-02
Accepted date: 2021-05-07
Online published: 2021-05-07
胡滨滨, 薛治慧, 张翠 . 植物小RNA荧光原位杂交实验方法[J]. 植物学报, 2021 , 56(3) : 330 -338 . DOI: 10.11983/CBB21057
Small RNAs are a type of small nucleotide molecules that are essential for plant growth and development, playing a key role in variety of life processes and in response to stresses. Research on the location of small RNAs could discover their functions in plants. Small RNA FISH is a qualitative or semi-quantitative analysis of small RNA in organisms by fluorescence detection technology. At present, this technology has been widely used in animals, but it is still less applied in plants. This article introduces the specific operation procedures and attentions based on ultra-high resolution microscopy that combines locked nucleic acid (LNA) probe in situ hybridization with immunofluorescence. This protocol can be used to detect the expression and localization of small RNA in plant tissues.
Key words： fluorescent in situ hybridization; small RNA; plants
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