植物转录因子与DNA互作研究技术
收稿日期: 2020-04-04
录用日期: 2020-06-28
网络出版日期: 2020-06-29
基金资助
国家自然科学基金(31870213)
Methods for Examining Transcription Factor-DNA Interaction in Plants
Received date: 2020-04-04
Accepted date: 2020-06-28
Online published: 2020-06-29
杨立文,刘双荣,李玉红,林荣呈 . 植物转录因子与DNA互作研究技术[J]. 植物学报, 2020 , 55(4) : 468 -474 . DOI: 10.11983/CBB20057
Transcription affects the growth and development of plants through regulating the spatio-temporal expression of downstream genes. The interaction between transcription factors and DNA is a key section in the process of exploring transcriptional regulatory networks. In the past few years, researchers utilize yeast one hybrid (Y1H) and electrophoresis mobility shift assay (EMSA) to examine whether a transcription factor directly interacts with target DNA. In addition, transient luciferase activity assay provides a convenient method for researchers to test the regulation of transcription factors on downstream gene expression. In this paper, we elaborate the principles, methods, and advantages and limitations of Y1H, EMSA and transient luciferase activity assay, to provide technical references for exploring the transcription factor-DNA interactions.
Key words： transcription factor; DNA; Y1H; EMSA; transient luciferase activity assay
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