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植物学报 >

2019 , Vol. 54 >Issue 4: 509 - 514

DOI: https://doi.org/10.11983/CBB19101

技术方法

利用低温水浴鉴定水稻苗期耐寒性

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  • 中国科学院植物研究所, 植物分子生理学重点实验室, 北京 100093

收稿日期: 2019-05-29

  录用日期: 2019-06-11

  网络出版日期: 2019-06-11

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Identification of Chilling Tolerance of Rice Seedlings by Cold Water Bath

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  • Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China

Received date: 2019-05-29

  Accepted date: 2019-06-11

  Online published: 2019-06-11

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摘要

高效准确鉴定苗期耐寒性是水稻(Oryza sativa)耐寒研究的前提。基于流动水浴温度均一这一特性, 建立了一种恒温水浴鉴定水稻幼苗耐寒性方法。该方法中环境温度设定为20°C, 水浴温度设定为4°C。根据对2个水稻亚种不同材料的处理结果, 总结出几种常见品种的低温处理时间与存活率参考值, 并对操作过程中的一些注意事项进行了说明。

关键词： 水稻; 苗期; 耐寒; 低温水浴

本文引用格式

刘栋峰,唐永严,雒胜韬,罗伟,李志涛,种康,徐云远 . 利用低温水浴鉴定水稻苗期耐寒性[J]. 植物学报, 2019 , 54(4) : 509 -514 . DOI: 10.11983/CBB19101

Abstract

The premise of studies on rice chilling tolerance is to find an efficient and accurate way to evaluate chilling tolerance of rice seedling. In this study, we developed an efficient technology to evaluate the cold tolerance at seedling stage by using a constant temperature water bath, based on the characteristics of excellent temperature uniformity for circulation of water. In this method, the setting temperature of environment temperature and water bath was 20°C and 4°C, respectively. From the results of two subspecies (indica/xian and japonica/geng), we summarized the reference treatment time for different survival rate of cultivars. Some attention to the cold treatment procedure was also discussed.

Key words： rice; seedling; cold tolerance; low temperature water bath

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参考文献

[1] Andaya VC, Tai TH ( 2006). Fine mapping of the qCTS12 locus, a major QTL for seedling cold tolerance in rice. Theor Appl Genet 113, 467-475.
[2] Baba S, Takahashi Y ( 1956). Water and sand culture. In:Togari Y, Matsuo T, Hatamari M, Yamada N, Horada T, Suzuki N, eds. Sakumotsi-Shiken-ho (Laboratory Manual in Crop Science). Kyokai, Tokyo: Nogyo-Gijitsu. pp. 157-185.
[3] Bonnecarrère V, Borsani O, Díaz P, Capdevielle F, Blanco P, Monza J ( 2011). Response to photoxidative stress induced by cold in japonica rice is genotype dependent. Plant Sci 180, 726-732.
[4] Ma Y, Dai XY, Xu YY, Luo W, Zheng XM, Zeng DL, Pan YJ, Lin XL, Liu HH, Zhang DJ, Xiao J, Guo XY, Xu SJ, Niu YD, Jin JB, Zhang H, Xu X, Li LG, Wang W, Qian Q, Ge S, Chong K ( 2015). COLD1 confers chilling tolerance in rice. Cell 160, 1209-1221.
[5] Yoshida S, Forno DA, Cock JH, Gomez KA ( 1976). Laboratory Manual for Physiological Studies of Rice. Las Banos, Laguna: IRRI. pp. 62.
[6] Zhang X, Guo XP, Lei CL, Cheng ZJ, Lin QB, Wang JL, Wu FQ, Wang J, Wan JM ( 2011). Overexpression of SlCZFP1, a novel TFIIIA-type zinc finger protein from tomato, confers enhanced cold tolerance in transgenic Arabidopsis and rice. Plant Mol Biol Rep 29, 185-196.
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