为了解供氮水平对不同时期盐胁迫下水稻(Oryza sativa)叶片光合及叶绿素荧光特性的影响, 以2个北方常规粳稻(Oryza sativa subsp. japonica)品种为材料, 在5个氮水平下进行培养, 于分蘖期、孕穗期和抽穗期分别进行盐胁迫处理, 测定分析了水稻叶片光合及叶绿素荧光参数的变化。结果表明, 与对照相比, 盐胁迫下水稻叶片的净光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)和表观叶肉导度(AMC)均显著降低, 在分蘖期、孕穗期和抽穗期分别以2N、1N和1/2N水平下降低的百分率最小; 气孔限制值(Ls)则显著增加, 分别以2N、1N和1/2N水平下增加的百分率最大。盐胁迫下, 与对照相比, PSII的实际光合效率(ΦPSII)、表观光合量子传递效率(ETR)和光化学淬灭(qP)均显著降低, 在分蘖期、孕穗期和抽穗期分别以2N、1N和1/2N水平下降低的百分率最小; 非光化学淬灭(NPQ)呈增加的变化趋势, 与对照相比, 分别以2N、1N和1/2N水平下增加的百分率最小。以上结果说明盐胁迫下水稻孕穗后, 供氮水平适量降低有利于减缓叶片光合作用的下降, 提高其抵御盐害能力。
徐晨, 刘晓龙, 李前, 凌凤楼, 武志海, 张治安
. 供氮水平对盐胁迫下水稻叶片光合及叶绿素荧光特性的影响[J]. 植物学报, 2018
, 53(2)
: 185
-195
.
DOI: 10.11983/CBB17063
We aimed to understand the influence of nitrogen levels on photosynthetic characteristics and chlorophyll fluorescence characteristics of rice leaf in different periods under salt stress. With two conventional japonica rice varieties from the north used as materials, the effect of five nitrogen levels were studied. Salt stress was applied at tillering, booting and heading. We studied the change in photosynthesis and chlorophyll fluorescence parameters of rice leaf. The net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr) and apparent mesophyll conductance (AMC) were significantly reduced under salt stress as compared with controls. The percentage decrease of Pn, Gs, Tr and AMC during tillering, booting and heading was minimum of 2N, 1N and 1/2N levels. The stomatal limitation (Ls) of rice leaf was significantly increased under salt stress. The percentage increase in Ls during tillering, booting and heading was maximum of 2N, 1N and 1/2N levels. The PSII actual photosynthetic efficiency (ΦPSII), apparent photosynthetic quantum transmission efficiency (ETR) and photochemical quenching (qP) were significantly reduced under salt stress as compared with controls. The percentage decrease in ΦPSII, ETR and qP during tillering, booting and heading was minimum of 2N, 1N and 1/2N levels. The non-photochemical quenching (NPQ) was increased under salt stress. The percentage increase in NPQ during tillering, booting and heading was minimum of 2N, 1N and 1/2N levels as compared with controls. A moderate amount of reducing nitrogen levels was helpful to slow the decline of leaf photosynthesis and to improve the ability to resist salt injury after booting stage of rice under salt stress.
[1] 高芸，程智慧，孟焕文. NaCl处理对番茄幼苗光合作用和叶绿素荧光的影响[J]. 干旱地区农业研究，2008， 26，194-199.
[2] 葛江丽，石 雷，谷卫彬，唐宇丹，张金政，姜闯道，任大明. 盐胁迫条件下甜高粱幼苗的光合特性及光系统II功能调节[J]. 作物学报，2007，33，1272-1278.
[3] 李敏，张洪程，李国业，魏海燕，殷春渊，马群，杨雄. 水稻氮效率基因型差异及其机理的研究进展[J]. 核农学报,2011,25，1057-1063.
[4] 刘晓龙，徐晨，徐克章，崔菁菁，安久海，凌风楼，张治安，武志海. 盐胁迫对水稻叶片光合作用和叶绿素荧光特性的影响[J]. 作物杂志,2014,2，88-92.
[5] 刘晓龙，徐晨，徐克章，崔菁菁，张治安，凌风楼，安久海，武志海. 不同供氮水平下盐胁迫对水稻光合特性和某些生理特性的影响[J]. 华南农业大学学报,2015, 36，6-12.
[6] 宁建凤，郑青松，杨少海，邹献中，孙丽丽，陈 勇. 高盐胁迫对罗布麻生长及离子平衡的影响[J]. 生态学报,2010,21，325-330.
[7] 宁建凤，郑青松，邹献中，孙丽丽，姚瑶，陈勇，巫金龙，魏岚. 罗布麻对不同浓度盐胁迫的生理响应[J]. 植物学报,2010, 45，689-697.
[8] 孙璐，周宇飞，李丰先，肖木辑，陶冶，许文娟，黄瑞冬. 盐胁迫对高粱幼苗光合作用和荧光特性的影响[J]. 中国农业科学,2012, 45，3265-3272.
[9] 王磊，隆小华，郝连香，刘兆普. 氮素形态对盐胁迫下菊芋幼苗PSII光化学效率及抗氧化特性的影响[J]. 草业科学,2012, 21，133-140.
[10] 吴家富，杨博文，向珣朝，许亮，颜李梅. 不同水稻种质在不同生育期耐盐鉴定的差异[J]. 植物学报,2017, 52，77-88.
[11] 徐晨，凌风楼，徐克章，武志海，刘晓龙，安久海，赵兰坡. 盐胁迫对不同水稻品种光合特性和生理生化特性的影响[J]. 中国水稻科学,2013,27，280-286.
[12] 尹海龙，田长彦. 氮调控对盐环境下甜菜功能叶光系统II荧光特性的影响[J]. 植物生态学报,2013, 37，122-131.
[13] 赵昕，吴雨霞，赵敏桂，何建新. NaCl 胁迫对盐芥和拟南芥光合作用的影响[J]. 植物学通报,2007, 24，154-160.
[14] Ali-Dinar H M，Ebert G，Lüdders P. Growth, Chlorophyll Content, Photosynthesis and Water Relations in Guava (Psidium guajava L.) Under Salinity and Different Nitrogen Supply. Gartenbauwissenschaft,1999,64，54–59.
[15] Ashraf M，Sultana R. Combination effect of NaCl salinity and nitrogen form on mineral composition of sunflower plants[J]. Biologia plantrum,2010,43，615-619.
[16] Cha-Um S，Nhung N T H，Kirdmanee C. Effect of mannitol-and salt-induced iso-osmotic stress on proline accumulation, photosynthetic abilities and growth characters of rice cultivars(Oryza Sativa L.Spp. Indica )[J]. Pakistan Journal of Botany,2010,42，927-941.
[17] Elizamar C S，Rejane J M C N，Francisco P A，Natoniel F M，André D A N. Physiological responses to salt stress in young umbu plants[J]. Environmental and Experimental Botany,2008,63，147–157.
[18] Manzer H S，Firoz M，M Nasir K，Al-Whaibi M H，Bahkali Ali H A. Nitrogen in Relation to Photosynthetic Capacity and Accumulation of Osmoprotectant and Nutrients in Brassica Genotypes Grown Under Salt Stress[J]. Agricultural Sciences in China,2010,9，671-680.
[19] Munns R，Tester M. Mechanisms and salinity tolerance[J]. Plant Biol,2008,59，651-681.
[20] Parida A K，Das A B. Salt tolerance and Salinity effects on plants: a review[J]. Ecotoxicology and environmental safety,2005,60，324-349.
[21] Silveira J A G，Melo A R B，Viégas R A，Oliveira J T A. Salinity-induced effect on nitrogen assimilation related to growth in cowpea plants[J]. Environmental and Experimental Botany,2001,46，171-179.
[22] Soussi M，Oca?a A，Lluch C. Effects of salt stress on growth, photosynthesis and nitrogen fixation in chick-pea(Cicer arietinum L.)[J]. Journal of Experimental Botany,1998,49，1329-1337.
[23] Summart J，Thanonkeo P，Panichajakul S，Prathepha P，McManus M T. Effect of salt stress on growth, inorganic ion and proline accumulation in Thai aromatic rice, Khao Dawk Mali 105, callus culture[J]. African Journal of Biotechnology,2010,9，145-152.
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