拟南芥谷氧还蛋白GRXC9负调控叶片大小
收稿日期: 2016-11-04
录用日期: 2017-03-06
网络出版日期: 2017-07-10
基金资助
广东省科技计划(No.2015B020231009, No.2015B020202007, No.2016A020208013, No.2015A020209156)
GRXC9 Negatively Regulates Leaf Size in Arabidopsis
Received date: 2016-11-04
Accepted date: 2017-03-06
Online published: 2017-07-10
谷氧还蛋白(GRX)是一类以CXXC/S基序为活性位点的小分子热稳定蛋白, 参与多种谷胱甘肽依赖的氧化还原反应。通过对该家族的GRXC9基因进行克隆、表达、亚细胞定位及功能分析, 结果表明, GRXC9基因表达无组织特异性, 在拟南芥(Arabidopsis thaliana)的根、茎、叶、花和角果中均能表达, 此结果与GUS显色结果基本一致。GRXC9-GFP定位于细胞质和细胞核中, 过表达GRXC9的株系叶片明显小于野生型; 进一步观察发现, 其叶片栅栏细胞明显变小, 而细胞总数与野生型差距不大。叶片大小相关基因的表达分析结果表明, 过表达株系中AN、LNG1和LNG2的表达量明显下降, 说明GRXC9可能通过抑制这些基因的表达从而导致叶片短小。综上所述, GRXC9可能在调控叶片发育方面发挥关键作用。
黄淦, 王潇, 金学锋, 王小菁, 王亚琴 . 拟南芥谷氧还蛋白GRXC9负调控叶片大小[J]. 植物学报, 2017 , 52(5) : 550 -559 . DOI: 10.11983/CBB16208
Glutaredoxin (GRX), a kind of small molecule and heat-stable protein with a CXXC/S motif as an active site, is involved in a variety of glutathione-dependent redox reactions. In this study, we performed cloning and expression, subcellular localization and functional analysis of GRXC9. GRXC9 could be detected in tissues and organs such as root, stem, leaf, flower and silique, which was consistent with GUS assay. GRXC9-GFP was localized in the nucleus and cytoplasm. GRXC9 overexpression lines showed smaller leaves than the wild type, and further observations showed smaller palisade cells in overexpression lines than the wild type, but the total number of cells was similar. The expression of the leaf size-related genes AN, LNG1 and LNG2 was significantly decreased in overexpression lines, so GRXC9 plays a negative role by inhibiting their expression and inducing small leaves. Taken together, GRXC9 may play a crucial role in regulating leaf development.
Key words： glutaredoxin; GRXC9; leaf size; negative regulation
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