粳稻子预44抗LP11稻瘟病菌基因Pizy6(t)的定位
# 共同第一作者
收稿日期: 2016-06-07
录用日期: 2016-07-29
网络出版日期: 2017-01-23
基金资助
国家重点研发计划(No.2016YFD0100601)和国家自然科学基金(No;U1302261, No.31160223)
Mapping of Pizy6(t), a Gene Conferring Resistance to the Rice Blast Strain LP11, in Oryza sativa subsp. japonica Cultivar Ziyu44
# Co-first authors
Received date: 2016-06-07
Accepted date: 2016-07-29
Online published: 2017-01-23
稻瘟病是世界范围内严重威胁水稻(Oryza sativa)生产可持续发展的主要病害之一, 每年造成10%-30%的水稻产量损失。抗瘟水稻品种的培育和育种利用是解决稻瘟病危害最经济有效的方法。对新的致病性菌株进行分离和筛选是定位与克隆抗病新基因及抗病育种的基础。选择分离自不同稻瘟病发生重灾区的单孢菌株, 对广谱抗瘟水稻子预44和感病水稻江南香糯进行致病性鉴定, 筛选出两材料间致病性差异明显的5个菌株; 进一步利用子预44、湘资3150、9311、日本晴、丽江新团黑谷、中花11、TP309和江南香糯8个抗瘟性不同的水稻材料, 对筛选的菌株进行致病性鉴定。结果显示, LP11能使广谱抗瘟籼稻湘资3150严重发病, 推测其很可能是新进化出来的强致病菌株。利用子预44和江南香糯杂交构建的F2群体进行抗性遗传分析, 结果表明子预44对LP11菌株的抗性是由单显性基因控制。利用SSR分子标记和图位克隆方法在子预44中定位了1个抗稻瘟病基因Pizy6(t)。研究结果不仅为抗病相关研究提供了有价值的新菌株, 而且为子预44中抗稻瘟病基因Pizy6(t)的克隆奠定了基础。
胡朝芹, 刘剑宇, 王韵茜, 杨睿, 汪秉琨, 何月秋, 曾千春, 罗琼 . 粳稻子预44抗LP11稻瘟病菌基因Pizy6(t)的定位[J]. 植物学报, 2017 , 52(1) : 61 -69 . DOI: 10.11983/CBB16126
Rice blast, caused by Magnaporthe oryzae infection, is one of the major diseases threatening the sustainable development of rice (Oryza sativa) production worldwide. Between 10% and 30% of the annual rice harvest is lost due to infection by the fungus. Development and breeding utilization of rice-resistant resources are considered the most effective and economic means to control rice blast. Isolating new virulent blast strains from the pathotype Oryza is the basis for cloning novel optimal genes resistant to rice blast and rice breeding for disease resistance. In the present study, we isolated and screened blast strains and identified new genes resistant to rice blast. Overall, 5 strains with significantly different pathogenicity between Ziyu44 and Jiangnanxiangnuo (JNXN) were identified. Eight rice varieties, including Ziyu44, Xiangzi3150, 9311, Nipponbare, Lijiangxintuanheigu (LTH), Zhonghua11, JNXN and TP309, were challenged with the 5 blast strains by spray-inoculation at seedling stage. Xiangzi3150, a variety with broad-spectrum blast resistance, showed obvious attacks after inoculation with LP11, so LP11 is probably a newly emerged strong virulent strain. The F2 progenies derived from the cross between Ziyu44 and JNXN were inoculated with the LP11; genetic analysis and gene mapping indicated that the resistance phenotype of Ziyu44 to LP11 was controlled by a single dominant gene, and the resistance gene Pizy6(t) was located on rice chromosome 6. These results provided valuable blast strains for genetic studies of disease resistance and laid the foundation for Pizy6(t) gene cloning in Ziyu44.
| [1] | 樊琳琳, 姚波, 刘志涛, 曾千春, 罗琼 (2015). 子预44中抗稻瘟病基因Pi-zy3(t)的定位. 分子植物育种 13, 961-967. |
| [2] | 胡标林, 谢建坤, 包劲松, 余守武, 张铮 (2006). 籼稻9311辐射突变体的分离与鉴定. 分子植物育种 4, 181-188. |
| [3] | 黄红梅, 肖应辉, 黄玲, 奉光平, 燕玮婷, 戴良英, 王国梁, 刘二明 (2011). 湘资3150微效抗瘟性基因鉴定. 植物病理学报 41, 509-515. |
| [4] | 李书, 李权, 樊琳琳, 沙莎, 曾千春, 罗琼 (2014). 高原粳稻子预44中三个稻瘟病抗性基因的假基因化分子标记鉴定. 分子植物育种 12, 219-225. |
| [5] | 温小红, 谢明杰, 姜健, 杨宝灵, 邵艳龙, 何伟, 刘丽, 赵毅 (2013). 水稻稻瘟病防治方法研究进展. 中国农学通报 29, 190-195. |
| [6] | 伍尚忠, 朱小源, 刘斌, 杨祁云, 张少红, Leung H (2004). 籼稻品种三黄占2号的稻瘟病持久抗性评价与遗传分析. 中国农业科学 37, 528-534. |
| [7] | 袁桂荣, 甘贤友 (1987). 一种有效的真菌单孢分离法. 河南职技师院学报 15, 70-71. |
| [8] | 张锦文, 洪汝科, 范静华, 张祎颖, 曾千春, 罗琼 (2011). 一份云南地方稻广谱持久抗稻瘟病初步分析. 西南农业学报 24, 1323-1326. |
| [9] | 张锦文, 谭亚玲, 洪汝科, 范静华, 罗琼, 曾千春 (2009). 高原粳稻子预44抗稻瘟病基因遗传分析和定位. 中国水稻科学 23, 31-35. |
| [10] | 张书建, 何月秋 (2003). 介绍一种简单的真菌单孢子分离法. 云南农业大学学报 18, 315-316. |
| [11] | 赵家铭, 张丽霞, 郑文静 (2014). 稻瘟病抗病基因定位及克隆研究进展. 辽宁农业科学 2, 47-49. |
| [12] | 周镕, 王波, 杨睿, 李书, 樊琳琳, 曾千春, 罗琼 (2015). 粳稻子预44中稻瘟病数量抗性位点分析. 植物学报 50, 691-698. |
| [13] | 朱旭东, 陈红旗, 罗达, 张建军, 方红民, 闵绍楷 (2003). 水稻中花11辐射突变体的分离与鉴定. 中国水稻科学 17, 205-210. |
| [14] | 邹征欧, 阳春瑜, 李闻名, 杨立明 (2016). 衡阳市水稻生产的现状与发展对策. 作物研究 2, 186-189. |
| [15] | Chen J, Shi YF, Liu WZ, Chai RY, Fu YP, Zhuang JY, Wu |
| [16] | JL (2011). A Pid3 allele from rice cultivar Gumei2 confers resistance to Magnaporthe oryzae. J Genet Genomics 38, 209-216. |
| [17] | Cheng SH, Cao LY, Zhuang JY, Chen SG, Zhan XD, Fan YY, Zhu DF, Peng SB (2007). Super hybrid rice breeding in China: achievements and prospects. J Integr Plant Biol 49, 805-810. |
| [18] | Guo CJ, Sun XG, Chen X, Yang SH, Li J, Wang L, Zhang XH (2016). Cloning of novel rice blast resistance genes from two rapidly evolving NBS-LRR gene families in rice.Plant Mol Biol 90, 95-105. |
| [19] | Huang J, Si W, Deng QM, Li P, Yang SH (2014). Rapid evolution of avirulence genes in rice blast fungus Magnaporthe oryzae.BMC Genet 15, 45. |
| [20] | International Rice Genome Sequencing Project (2005). The map-based sequence of the rice genome.Nature 436, 793-800. |
| [21] | Murray MG, Thompson WF (1980). Rapid isolation of high molecular weight plant DNA.Nucleic Acids Res 8, 4321-4325. |
| [22] | Sharma TR, Rai AK, Gupta SK, Vijayan J, Devanna BN, Ray S (2012). Rice blast management through host-plant resistance: retrospect and prospects.Agric Res 1, 37-52. |
| [23] | Tanweer FA, Rafii MY, Sijam K, Rahim HA, Ahmed F, Latif MA (2015). Current advance methods for the identification of blast resistance genes in rice.Plant Biol Pathol 338, 321-334. |
| [24] | Waller JM, Qu SH (1985). Rice Diseases (2nd). London: Common Wealth Agricultural Bureau. pp.109-201. |
| [25] | Xu X, Lv QM, Shang JJ, Pang ZQ, Zhou ZZ, Wang J, Jiang GH, Tao Y, Xu Q, Li XB, Zhao XF, Li SG, Xu JC, Zhu LH (2014). Excavation of Pid3 orthologs with differential resistance spectra to Magnaporthe oryzae in rice resource. PLoS One 9, e93275. |
| [26] | Yang SH, Li J, Zhang XH, Zhang QJ, Huang J, Chen JQ, Hartl DL, Tian DC (2013). Rapidly evolving R genes in diverse grass species confer resistance to rice blast disease.Proc Natl Acad Sci USA 110, 18572-18577. |
| [27] | Zhou B, Qu SH, Liu GF, Dolan M, Sakai H, Lu GD, Bellizzi M, Wang GL (2006). The eight amino-acid differences within three leucine-rich repeats between Pi2 and Piz-t resistance proteins determine the resistance specificity to Magnaporthe grisea.Mol Plant Microbe Interact 19, 1216-1228. |
/
| 〈 |
|
〉 |
首页