水稻矮化基因WLD1的图位克隆
# 共同第一作者
收稿日期: 2016-01-15
录用日期: 2016-04-29
网络出版日期: 2017-01-23
基金资助
农业部重大转基因专项(No.2014Ex08001-003)
Gene Mapping of a Dwarf Gene WLD1 in Rice
# Co-first authors
Received date: 2016-01-15
Accepted date: 2016-04-29
Online published: 2017-01-23
水稻(Oryza sativa)矮化是与光合效率及产量等密切相关的重要农艺性状。发掘更多的水稻矮秆资源, 不仅能够进一步加深对水稻株高分子遗传机制的认识, 而且还能为水稻新品种培育提供新的种质资源。在水稻T-DNA插入突变体库中筛选到1个矮化、宽叶小粒突变体(wld1)。经图位克隆将WLD1基因定位在第5号染色体长臂, 位于分子标记InDel37与InDel48之间, 基因编号为LOC_Os05g32270, 属于AP2转录因子家族。该基因第6外显子处胸腺嘧啶缺失, 造成转录提前终止。石蜡切片观察结果显示, 茎部第2节间横向细胞数目增加, 而纵向细胞数目未变。RT-PCR检测结果表明, LOC_Os05g32270在突变体wld1中不表达, 造成功能缺失。该基因与已报道的水稻OsSMOS1 (SMALL ORGAN SIZE1)为等位基因。水稻突变体wld1的矮秆遗传效应可直接应用于育种中。该研究结果进一步明确了突变体wld1的表型特征与遗传基础, 为解析其参与的信号途径提供参考。
王文乐, 冯丹, 吴金霞, 张治国, 路铁刚 . 水稻矮化基因WLD1的图位克隆[J]. 植物学报, 2017 , 52(1) : 54 -60 . DOI: 10.11983/CBB16010
Dwarf rice (Oryza sativa) is the most important agronomic trait closely related to photosynthetic efficiency and yield. Exploring more dwarf resources can deepen our understanding of the genetic mechanisms of plant height and provide new germplasm for cultivating new varieties. A wide-leaf, wide-stem, small-kernel and dwarf mutant, wld1, was identified from the rice T-DNA insertion mutant library. We used map-based cloning to locate the gene on rice chromosome 5 between the markers InDel37 and InDel48, with 91 kb physical distance. The mutant wld1 resulted from deletion of the base T in LOC_Os05g32270, which causes a reading error and early termination of the peptide chain encoding. We used microscopy to observe the top-second internal structure of vegetative organs of the mutant and the wild type in paraffin sections. In the wld1, cells were significantly increased in size in cross-sections but reduced in size in longitudinal sections. The expression of LOC_Os05g32270 was completely inactivated. The gene is an allele of the reported rice gene OsSMOS1 (SMALL ORGAN SIZE1). The agronomic properties of wld1 are good (lodging) and it can be directly applied to practice. In this study, we defined the inheritance and phenotype characteristics of this mutant, which lays the foundation for further analysis of the signaling pathway.
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