筛选堇叶紫金牛(Ardisia violacea)野生优株, 以其当年新发带休眠腋芽茎段为外植体, 通过启动培养、丛生芽诱导增殖、壮苗培养、生根培养和炼苗移栽等过程建立其组培快繁技术体系。研究结果表明, 最佳启动培养基为MS+0.80 mg·L^-1 KT+0.10 mg·L^-1 NAA+0.10 mg·L^-1 IBA, 腋芽萌发率达92.60%; 最佳丛生芽诱导增殖培养基为MS1+0.50 mg·L^-1 TDZ+0.10 mg·L^-1 NAA, 平均增殖系数达8.60; 最佳壮苗培养基为MS+1.00 mg·L^-1 KT+0.50 mg·L^-1 NAA; 最佳生根培养基为1/2MS+2.00 mg·L^-1 IBA+1.00 mg·L^-1 NAA+1.00 mg·L^-1 AC, 平均生根率达98.70%; 采用松鳞和泥炭(2:1, v/v)作为炼苗基质, 炼苗成活率可达85.30%。实验成功建立了堇叶紫金牛高效组培快繁技术体系, 经验证该体系能够满足规模化生产的需求。

Shoot segments with axillary buds from young branches of wild Ardisia violacea with excellent characters were cultured in vitro. Through bud induction, multiple shoots proliferation, subculture, rooting and transplantation, a regeneration system was established. The optimal bud induction medium was MS+0.80 mg?L^-1 KT+0.10 mg?L^-1 NAA+0.10 mg?L^-1 IBA, and the germination frequency of axillary buds reached 92.60%; the optimal multiple shoot proliferation medium was MS1+0.50 mg?L^-1 TDZ+0.10 mg?L^-1 NAA, and the average proliferation coefficient reached 8.60; the optimal subculture medium was MS+1.00 mg?L^-1 KT+0.50 mg?L^-1 NAA; the optimal rooting medium was 1/2MS+2.00 mg?L^-1 IBA+1.00 mg?L^-1 NAA+1.00 mg?L^-1 AC, and the average frequency of shoot rooting reached 98.70%. Using pine bark:peat (2:1, v/v) as a matrix, the transplant survival rate could reach 85.30%. We established a high-frequency regeneration system for A. violacea that could meet the needs of large-scale production.