研究报告

大豆植株不同冠层种子活力及其萌发中抗氧化酶活性

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  • 吉林农业大学农学院, 长春 130118

? 共同第一作者

收稿日期: 2015-01-18

  录用日期: 2015-04-14

  网络出版日期: 2016-02-01

基金资助

基金项目: 国家自然科学基金(No.31171478)、国家863计划(No.2011AA105)和吉林省自然科学基金(No.201215178)

Seed Vigor and Antioxidant Enzyme Activities During Germination in Different Canopies of Soybean

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  • College of Agronomy, Jilin Agricultural University, Changchun 130118, China

? These authors contributed equally to this paper

Received date: 2015-01-18

  Accepted date: 2015-04-14

  Online published: 2016-02-01

摘要

以吉农15号和吉农24号大豆(Glycine max)品种为材料, 根据植株高度平均分为下部、中下部、中部、中上部和上部5个冠层, 分析不同冠层种子百粒重、种子活力及萌发7天子叶中抗氧化酶活性和丙二醛含量的变化。结果表明, 随着植株冠层的升高, 大豆百粒重呈先略上升后逐渐下降的趋势, 上部冠层种子的百粒重最小。大豆植株下部与中下部冠层种子的发芽率和发芽势较高; 并且该两层的种子活力指数显著高于中上部与上部冠层, 种子萌发后幼苗的茎粗也显著大于中上部及上部冠层幼苗。下部与中下部冠层种子萌发时子叶中的抗氧化酶活性高于上部, 而丙二醛含量低于上部, 说明该两层的大豆种子活力高, 萌发后幼苗健壮且子叶新陈代谢旺盛, 是优选良种的最佳冠层。

本文引用格式

陈思羽, 刘鹏, 朱末, 夏冬冬, 李亮, 徐克章, 陈展宇, 张治安 . 大豆植株不同冠层种子活力及其萌发中抗氧化酶活性[J]. 植物学报, 2016 , 51(1) : 24 -30 . DOI: 10.11983/CBB15014

Abstract

Jinong 15 and Jinong 24 were divided into 5 canopies by plant height—lower, lower-middle, middle, up- per-middle and upper—for analyzing 100-seed weight, seed vigor, changes in antioxidant enzyme activities and MDA content with germination for 7 d in different canopies of soybean cotyledon. Soybean 100-seed weight increased slightly and then decreased gradually with increasing canopy, with the upper ones being the lightest. The germination rate and germination potential were higher in the low and lower-middle heights, and their vigor indexes were significantly higher than in the upper-middle and upper heights. The stem diameter of seed germination seedlings were significantly greater in the lower and lower-middle heights than in the upper-middle and upper heights. The antioxidant enzyme activities of the cotyledon during seed germination were higher in the lower and lower-middle heights than the upper height, but the MDA content was less than in the upper height. The low and lower-middle part was the best canopy because the seed vigor was high, seedlings were robust, and cotyledon metabolism was vigorous.

参考文献

1 曹栋栋 (2010). 多胺对超甜玉米种子发育过程种子质量和萌发的调控作用研究. 博士论文. 杭州: 浙江大学. pp. 4-24.
2 陈禅友 (1992). 豇豆种子活力研究进展. 种子 5, 45-47.
3 冯引弟, 徐克章, 季平, 张鹏, 徐晨, 李大勇 (2014). 新老大豆品种不同节位叶片性状及其与籽粒产量的相关性研究. 西北农林科技大学学报 42, 61-68.
4 金剑, 刘晓冰, 王光华 (2004). 不同熟期大豆R4-R5期冠层某些生理生态性状与产量的关系. 中国农业科学 37, 1293-1300.
5 李合生 (2006). 现代植物生理学(第3版). 北京: 高等教育出版社. pp. 324-325.
6 刘军, 黄上志, 傅家瑞, 汤学军 (2001). 种子活力与蛋白质关系的研究进展. 植物学通报 18, 46-51.
7 苗以农, 朱长甫, 姜艳秋, 刘学军 (1989). 大豆不同生育期不同节位叶片的比叶重及叶绿素和全氮含量. 中国油料 4, 44-48.
8 钱森和, 厉荣玉, 杨超英, 汤斌, 薛正莲 (2009). PSB对大豆种子活力及其生物学特性影响的研究. 种子 28, 25-28.
9 孙卓韬, 董钻 (1986). 大豆株型、群体结构与产量关系的研究第二报大豆群体冠层的荚粒分布. 大豆科学 5, 91-102.
10 谢特立, Tekrony DM (2005). 品种、种植密度与结荚位对大豆种子活力的影响研究. 中国生态农业学报 13, 69-72.
11 杨晓杰, 孙志琳 (2003). 阿司匹林和复方新诺明对大豆种子萌发及活力的影响. 植物生态学报 27, 667-671.
12 杨永青, 汪晓峰 (2004). 种子活力与生物膜的研究现状. 植物学通报 21, 641-648.
13 张治安, 陈展宇 (2008). 植物生理学实验指导. 长春: 吉林大学出版社. pp. 100-111, 180-193.
14 张治安, 王振民, 徐克章 (2005). Cd胁迫对萌发大豆种子中活性氧代谢的影响. 农业环境科学学报 24, 670-673.
15 庄炳昌, 徐豹 (1990). 大豆不同节位种子蛋白质脂肪及其组分的分布. 中国油料 3, 42-44.
16 庄波, 徐克章, 杜双洋, 李大勇, 张治安 (2010). 新、老大豆品种冠层产量和光合作用的比较. 华南农业大学学报 31, 6-9.
17 Alscher RG, Erturk N, Health LS (2002). Role of superoxi- de dismutases (SODs) in controlling oxidative stress in plants.J Exp Bot 53, 1331-1341.
18 Chen JY, Wen PF, Kong WF, Pan QH, Zhan JC, Li JM, Wan SB, Huang WD (2006). Effect of salicylic acid on phenylpropanoids and phenylalanine ammonia-lyase in harvested grape berries.Postharvest Biol Tec 40, 64-72.
19 Dong JE, Wan GW, Liang ZS (2010). Accumulation of salicylic acid-induced phenolic compounds and raised ac- tivities of secondary metabolic and antioxidative enzymes in Salvia miltiorrhiza cell culture.J Biotechnol 148, 99-104.
20 Ertsey DK, Muschick M (2010). Seed Testing International No.139. .
21 Escalante EE, Wilcox JR (1993). Variation in seed protein among nodes of determinate and indeterminate soybean near-isolines.Crop Sci 33, 1166-1168.
22 James EB (2004). Soybean cultivar differences on light in- terception and leaf area index during seed filling. Agron J 96, 305-311.
23 Shim IS, Momose Y, Yamamoto A, Kim DW, Usui K (2003). Inhibition of catalase activity by oxidative stress and its relationship to salicylic acid accumulation in plants.Plant Growth Regul 39, 285-292.
24 Takahashi MA, Asada K (1983). Superoxide anion permeability of phospholipids membranes and chloroplast thylakoids.Arch Biochem Biophys 226, 558-566.
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