荚果蕨绿色球状体对60Coγ射线的辐射敏感性
- 1中国科学院植物研究所北方资源植物重点实验室/北京植物园, 北京 100093
2云南省农业科学院花卉研究所, 国家观赏园艺工程技术研究中心, 昆明 650205;
3中国科学院庐山植物园, 九江 332900
收稿日期: 2014-08-06
修回日期: 2014-12-14
网络出版日期: 2015-10-09
Radiosensitivity of Green Globular Bodies of Matteuccia struthiopteris Exposed to 60Coγ Radiation
- 1The Key Laboratory of Northern Plant Resources/Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;
2National Engineering Research Center for Ornamental Horticulture, Flower Research Institute, Yunnan Academy of Agricultural Science, Kunming 650205, China;
3Lushan Botanical Garden, Chinese Academy of Sciences, Jiujiang 332900, China
Received date: 2014-08-06
Revised date: 2014-12-14
Online published: 2015-10-09
摘要
以荚果蕨(Matteuccia struthiopteris)绿色球状体(GGB)为辐照材料, 使用不同剂量的60Coγ射线进行辐照处理, 采用辐射诱变和组织培养技术相结合的方式进行荚果蕨GGB的辐射敏感性研究。结果表明, 荚果蕨GGB的存活率与60Coγ射线辐照剂量之间存在显著的线性关系, 线性回归方程为: y=–0.797x+104.719, 计算得到半致死剂量约为69 Gy。随着辐照剂量的增加, 荚果蕨GGB的增殖和分化能力下降且分化时间延长。GGB 60Coγ辐射的表型损伤表现为颜色变深、单个绿色颗粒死亡、增殖及分化受到抑制; 细胞学损伤表现为微核、细胞核解体和胚性细胞减少。
本文引用格式
余蓉培, 李杨, 李东, 詹选怀, 石雷 . 荚果蕨绿色球状体对60Coγ射线的辐射敏感性[J]. 植物学报, 2015 , 50(5) : 565 -572 . DOI: 10.11983/CBB14141
Abstract
Combining tissue culture with radiation mutation techniques, we investigated the radiosensitivity of green globular bodies (GGB) of Matteuccia struthiopteris by exposing it to various doses of 60Coγ radiation. The significant linearity between GGB survival and doses of 60Coγ could be expressed as y=–0.797x+104.719, LD50=69 Gy. The multiplication and differentiation of GGB was decreased and the differentiation period was prolonged with increasing radiation doses. Phenotypic damage such as dark color, death of single green bodies, and cellular damage such as micronuclei, broken nuclei and decrease of embryonic cell after irradiation.