一种快速、无损大豆种子DNA提取方法的建立和应用

程文, 夏正俊, 冯献忠, 杨素欣

doi:10.11983/CBB15057

植物学报 >

2016 , Vol. 51 >Issue 1: 68 - 73

DOI: https://doi.org/10.11983/CBB15057

技术方法

一种快速、无损大豆种子DNA提取方法的建立和应用

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¹山东师范大学生命科学学院, 济南 250014
²中国科学院东北地理与农业生态研究所中国科学院大豆分子设计育种重点实验室, 长春 130102
³中国科学院东北地理与农业生态研究所中国科学院大豆分子设计育种重点实验室, 哈尔滨 150081

? 共同第一作者

收稿日期: 2015-03-31

录用日期: 2015-09-25

网络出版日期: 2016-02-01

基金资助

基金项目: 国家自然科学基金(No.31171571, No.91131008, No.31271743)

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A Rapid and Nondestructive Method for Soybean DNA Extraction and Its Application

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¹College of Life Sciences, Shandong Normal University, Jinan 250014, China
²Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China
³Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081, China

? These authors contributed equally to this paper

Received date: 2015-03-31

Accepted date: 2015-09-25

one_p">2015-09-25

Online published: 2016-02-01

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摘要

基因分型是进行植物基因功能的遗传分析和分子标记辅助育种的重要环节。该研究以大豆(Glycine max)成熟种子为材料, 建立了通过钻孔采集样品、快速提取DNA进行基因型鉴定的方法。用此方法, 一个熟练的工作人员可以在1个小时内完成120个样品的采集和DNA提取; 同时种子钻孔取样后, 不会对大豆种子的萌发造成影响。利用该方法获得的DNA可满足PCR扩增的要求。实验重复性好, 成功率在98%以上。这种快速且无损的大豆种子基因型鉴定方法可以用于鉴定杂交种子、品种纯度以及遗传分析等研究工作。

关键词： 大豆种子; DNA提取; 无损基因型鉴定

本文引用格式

程文, 夏正俊, 冯献忠, 杨素欣 . 一种快速、无损大豆种子DNA提取方法的建立和应用[J]. 植物学报, 2016 , 51(1) : 68 -73 . DOI: 10.11983/CBB15057

Abstract

Genotyping is an important work in plant genetic analysis and molecular marker-assisted breeding. This study established a simple method for genotyping soybean from mature seeds. By this method, a skilled worker can perform the sample collection and DNA extraction of 120 samples from soybean seeds in 1 h and the treated seeds can germinate normally. The DNA obtained by this method can meet the requirement for PCR amplification, and the success rate of PCR reaction is over 98%. This method can be widely used for identifying soybean hybrid seeds, seed purity and genotyping.

Key words： soybean seed; DNA extraction; nondestructive genotyping

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参考文献

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