研究报告
番茄环纹斑点病毒与马铃薯Y病毒复合侵染烟草的细胞病理特征
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- 云南省农业科学院生物技术与种质资源研究所, 云南省农业生物技术重点实验室, 农业部西南作物基因资源与种质创制重点实验室, 昆明 650223
收稿日期: 2014-09-04
修回日期: 2014-10-10
网络出版日期: 2014-11-21
基金资助
云南省创新团队;973前期专项
Cytopathological Characterization of Tobacco on Co-infection with Tomato zonate spot virus and Potato virus Y
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- Ministry of Agriculture, Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation, Yunnan Provincial Key Laboratory of Agricultural Biotechnology, Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223
Received date: 2014-09-04
Revised date: 2014-10-10
Online published: 2014-11-21
摘要
在自然情况下, 番茄环纹斑点病毒(TZSV)和马铃薯Y病毒(PVY)通常复合侵染同一植株。该文首次报道了TZSV和PVY复合侵染烟草(Nicotiana sp.)植株的细胞病理特征, 并与单独侵染进行了比较分析。复合侵染的烟草植株细胞中, TZSV病毒颗粒明显增多, 并聚集于囊膜内形成包涵体, 与PVY的风轮状及片层状内含体和病毒颗粒聚集体交叉分布于细胞质(内含线粒体明显增多)中, 线粒体、叶绿体和细胞核结构较完整; 两种病毒的颗粒、包涵体和内含体数量均较单一侵染增多, 且对寄主亚细胞结构的破坏较单一侵染为轻, TZSV和PVY及其与寄主的互作可能存在协生作用。
本文引用格式
方琦, 董家红, 郑宽瑜, 张仲凯 . 番茄环纹斑点病毒与马铃薯Y病毒复合侵染烟草的细胞病理特征[J]. 植物学报, 2014 , 49(6) : 704 -709 . DOI: 10.3724/SP.J.1259.2014.00704
Abstract
Tomato zonate spot virus (TZSV) and Potato virus Y (PVY) commonly co-infect the same plant under natural conditions. We compared the pathological characterization resulting from mixed and single infection of tobacco. Cells with mixed infection showed increased number of TZSV particles, and the particles clustered into the vesicles to form inclusion bodies, which cross-distributed in the cytoplasm with pinwheel and laminated inclusions and particles. The number of mitochondria was increased. The structure of mitochondria, chloroplasts, and the nucleus maintained relative integrity. The number of particles, inclusion bodies, and inclusions was increased but did not cause more severe damage on the subcellular structure when TZSV or PVY co-infected the same tobacco plant. A synergism may exist among TZSV, PVY and the plant host during co-infection.
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Kikkert M, Van Lent J, Storms M, Bodegom P, Kormelink R, Goldbach R (1999).Tomato spotted wilt virus particle morphogenesis in plant cells. J Virol 73, 2288-2297.
Malapi-Nelson M, Wen R-H, Ownley BH, Hajimorad MR (2009). Co-infection of soybean with Soybean mosaic virus and Alfalfa mosaic virus results in disease synergism and alteration in accumulation level of both viruses. Plant Dis 93, 1259-1264.
Untiveros M, Fuentes S, Salazar LF (2007). Synergistic interaction of Sweet potato chlorotic stunt virus (Crinivirus) with carla-, cucumo-, ipomo-, and potyviruses infecting sweet potato. Plant Dis 91,669-676.
Vance VB (1991). Replication of potato virus X RNA is altered in coinfections with potato virus Y. Virol 182, 486-494.
Wang Y, Gaba V, Yang J, Palukaitis P, Gal-On A (2002). Characterization of synergy between Cucumber mosaic virus and potyviruses in cucurbit hosts. Phytopathol 92,51-58.
洪健，李德葆，周雪平 (2001)． 植物病毒分类图谱. 北京: 科学出版社. pp. 98-114.
谢礼，吕明芳，王芳，季志仙，吴列洪，严成其，陈剑平 (2013).甘薯羽状斑驳病毒和甘薯褪绿矮化病毒复合侵染甘薯引起的细胞病理学研究.电子显微学报 32，485-491.
张仲凯，方琦，丁铭，李停婷，彭潞波，张丽珍，李展，苏晓霞 (2004b).TSWV与TMV复合侵染大花蕙兰的细胞病理. 西南农业学报 17, 155-159.
张仲凯，方琦，董家红，丁铭，李婷婷，苏晓霞 (2005).番茄斑萎病毒侵染引起寄主细胞的质壁分离. 电子显微学报 24，423.
张仲凯，李毅 (2001). 云南植物病毒. 北京：科学出版社. pp. 17-18.
张仲凯，杨录明，方琦，彭潞波，汪继玲，普耀芳 (2004a).番茄斑萎病毒侵染烟草的细胞病理. 电子显微学报 23,349.
郑雪，刘春明，李宏光，张洁，董家红，张仲凯 (2013). 云南省红河州传播番茄斑萎病毒属病毒的蓟马种类及其寄主植物调查．中国植保导刊 33, 41-44.
郑瑛，洪健，祝建，陈集双 (2010). 应用高压冷冻-冷冻置换技术研究受Potyvirus侵染的寄主细胞超微结构.电子显微学报 29, 146-151.
Bag S, Mitter N, Eid S, Pappu HR (2012). Complementation between Two Tospoviruses Facilitates the Systemic Movement of a Plant Virus Silencing Suppressor in an Otherwise Restrictive Host. PLoS One 7(10): e44803
Cai J, Qin B, Wei X, Huang J, Zhou W, Lin B, Yao M, Hu Z, Feng Z, Tao X (2011). Molecular identification and characterization of Tomato zonate spot virus in tobacco in Guangxi, China. Plant Dis 95,1483.3.
Dong JH, Cheng XF, Yin YY, Fang Q, Ding M, Li TT, Zhang LZ, Su XX, McBeath JH, Zhang ZK (2008). Characterization of Tomato zonate spot virus, a new Tospovirus species in China. Arch Virol 153,855-864.
Dong JH, Zhang ZK, Yin YY, Cheng XF, Ding M and Fang Q (2010). Natural host ranges of Tomato zonate spot virus in Yunnan. J Insect Sci 10,12-13.
García-Cano E, Resende RO, Fernández-Mu?oz R, Moriones E (2006). Synergistic interaction between Tomato chlorosis virus and Tomato spotted wilt virus results in breakdown of resistance in tomato. Phytopathol 96,1263-1269.
Garcia-Marcos A, Pacheco R, Martiá?ez J, González-Jara P, Díaz-Ruíz JR, Tenllado F (2009). Transcriptional changes and oxidative stress associated with the synergistic interaction between Potato virus X and Potato virus Y and their relationship with symptom expression.Mol Plant-Microbe Interact 22,1431-1444.
Hoffmann K, Geske SM, Moyer JW (1998). Pathogenesis of tomato spotted wilt virus in peanut plants dually infected with peanut mottle virus. Plant Dis 82, 610-614.
Karyeija RF, Kreuze JF, Gibson RW, Valkonen JP (2000).Synergistic interactions of a potyvirus and a phloemlimited crinivirus in sweet potato plants．Virol 269: 26-36．
Kikkert M, Van Lent J, Storms M, Bodegom P, Kormelink R, Goldbach R (1999).Tomato spotted wilt virus particle morphogenesis in plant cells. J Virol 73, 2288-2297.
Malapi-Nelson M, Wen R-H, Ownley BH, Hajimorad MR (2009). Co-infection of soybean with Soybean mosaic virus and Alfalfa mosaic virus results in disease synergism and alteration in accumulation level of both viruses. Plant Dis 93, 1259-1264.
Untiveros M, Fuentes S, Salazar LF (2007). Synergistic interaction of Sweet potato chlorotic stunt virus (Crinivirus) with carla-, cucumo-, ipomo-, and potyviruses infecting sweet potato. Plant Dis 91,669-676.
Vance VB (1991). Replication of potato virus X RNA is altered in coinfections with potato virus Y. Virol 182, 486-494.
Wang Y, Gaba V, Yang J, Palukaitis P, Gal-On A (2002). Characterization of synergy between Cucumber mosaic virus and potyviruses in cucurbit hosts. Phytopathol 92,51-58.
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