研究报告

茄子种质遗传多样性及群体结构的SRAP分析

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  • 1 哈尔滨市农业科学院, 哈尔滨 150028
    2 湖北省农业科学院经济作物研究所, 武汉 430064

收稿日期: 2017-12-06

  录用日期: 2018-08-21

  网络出版日期: 2019-07-31

基金资助

国家重点研发计划(2016YFD0101703);哈尔滨市应用技术研究与开发项目(2016RQYY0020);黑龙江省应用技术研究与开发计划(CA15B103-1)

Genetic Diversity and Population Structure of Eggplant (Solanum melongena) Germplasm Resources Based on SRAP Method

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  • 1 Harbin Academy of Agricultural Sciences, Harbin 150028, China
    2 Institute of Economic Crops of Hubei Academy of Agricultural Sciences, Wuhan 430064, China

Received date: 2017-12-06

  Accepted date: 2018-08-21

  Online published: 2019-07-31

摘要

采用SRAP标记法对183份茄子(Solanum melongena)种质资源的遗传关系和群体结构进行分析。结果表明, 33对多态性SRAP引物组合共扩增出215条清晰稳定的多态性条带, 平均每对引物组合产生7条多态性条带。183份茄子种质的遗传相似系数介于0.276-0.813之间, 平均值为0.623, 表明茄子种质资源间遗传背景存在一定的差异。在遗传距离为0.345 6处可将183份茄子种质分为4组。通过群体结构分析可将种质划分为4个群体, 不同群体间的界限十分明显, 且群体间的基因渗透较高。

本文引用格式

谢立峰,李宁,李烨,姚明华 . 茄子种质遗传多样性及群体结构的SRAP分析[J]. 植物学报, 2019 , 54(1) : 58 -63 . DOI: 10.11983/CBB17237

Abstract

The genetic diversity and the population structure of 183 germplasm resources of eggplant were analyzed by using SRAP markers. Overall, 215 bands were generated with 7 binds per primer on average. The mean genetic similarity coefficient was 0.623 among the cultivars (0.276-0.813), which suggested genetic differentiation among the germplasm resources. The germplasm resources were divided into four groups with a similarity coefficient of 0.345 6. Introgression exists in the four groups based on population structure analysis. The boundaries among different groups are obvious, and gene introgression among varieties is high.

参考文献

1 冯英娜, 柳李旺, 刘卫东, 王倩, 崔群香 ( 2014). 茄子SSR遗传多样性及其农艺性状的关联分析. 江苏农业学报 30, 839-847.
2 何倚剑, 刘卫东, 柳李旺, 王倩, 崔群香 ( 2013). 茄子种质资源遗传多样性和群体结构分析. 南京农业大学学报 36(5), 13-20.
3 李宁, 姚明华, 焦春海, 李烨, 王飞 ( 2014). 亚洲及非洲茄子种质资源主要农艺性状的遗传多样性分析. 湖北农业科学 53, 5769-5774.
4 李涛, 黎振兴, 罗少波, 李植良, 徐小万 ( 2014). 东南亚茄子种质资源ISSR遗传多样性分析. 中国农学通报 30(25), 104-110.
5 刘丽华, 王立新, 赵昌平, 姚骥, 张风廷, 张华, 叶志杰, 秦志列, 郑用琏 ( 2009). 光温敏二系杂交小麦恢复系遗传多样性和群体结构分析. 中国生物化学与分子生物学报 25, 867-875.
6 毛伟海, 杜黎明, 包崇来, 胡天华, 朱琴妹, 胡海娇 ( 2006). 我国南方长茄种质资源的ISSR标记分析. 园艺学报 33, 1109-1112.
7 冉进, 宋明, 房超, 文明玲, 刘小俊, 刘独臣, 李跃建 ( 2007). 茄子(S. melongena L.)种质资源遗传多样性的RAPD分析. 西南农业学报 20, 694-697.
8 孙源文, 陈钰辉, 刘富中, 张映, 连勇 ( 2012). 基于SSR分子标记的栽培种茄子遗传多样性分析. 中国蔬菜 ( 22), 17-23.
9 王秋锦, 高杰, 孙清鹏, 杨爱珍, 赵福宽 ( 2007). 茄子品种遗传多样性的RAPD检测与聚类分析. 植物生理学报 43, 1035-1039.
10 An CF, Saha S, Jenkins JN, Ma DP, Scheffler BE, Kohel RJ, Yu JZ, Stelly DM ( 2008). Cotton ( Gossypium spp.) R2R3-MYB transcription factors SNP identification, phylogenomic characterization, chromosome localization, and linkage mapping.Theor Appl Genet 116, 1015-1026.
11 Falush D, Stephens M, Pritchard JK ( 2007). Inference of population structure using multilocus genotype data: dominant markers and null alleles. Mol Ecol Notes 7, 574-578.
12 Flint-Garcia SA, Thornsberry JM, Buckler ES ( 2003). Structure of linkage disequilibrium in plants. Annu Rev Plant Biol 54, 357-374.
13 Li G, Quiros CF ( 2001). Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica.Theor Appl Genet 103, 455-461.
14 Li HZ, Chen HY, Zhuang TM, Chen J ( 2010). Analysis of genetic variation in eggplant and related Solanum species using sequence-related amplified polymorphism markers.Sci Hortic 125, 19-24.
15 Perrier X, Flori A, Bonnot F (2003). Data analysis methods. In: Hamon P, Seguin M, Perrier X, eds. Genetic Diversity of Cultivated Tropical Plants. Montpellier: Enfield, Science Publishers. pp. 43-76.
16 Rohlf FJ ( 1997). NTSYS-pc-Numerical Taxonomy and Multivariate Analysis System Version 2.02e. Exeter Software. New York.
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