为建立一种更为简便且准确的水稻(Oryza sativa)硅含量测定方法, 对硅钼蓝分光光度法的多个实验条件进行了优化, 并对水稻样品的前处理——消解法和高压灭菌法进行了比较研究。结果表明, 还原剂为抗坏血酸、测定波长为600 nm、硅钼黄显色时间和硅钼蓝稳定时间分别为5和25分钟为最佳测定条件, 样品的前处理则是消解法优于高压灭菌法, 前者提取硅的效果好于后者。该优化方法为深入研究水稻和其它植物中硅的吸收和转运机制奠定了基础。

To establish an easier and more accurate method to determine silicon content in rice, several main parameters of silicon molybdenum-blue spectrophotometry were optimized in combination with 2 sample pretreatments, digestion and autoclaving. By using ascorbic acid as a reductant, with the color development time of the silicon molybdenum yellow and the stable time of silicon molybdenum blue for 5 and 25 min, respectively, optimal silicon content in rice was achieved at 600 nm wavelength by molybdenum-blue spectrophotometry. For sample pretreatment, the digestion method was more effective for silicon extraction than autoclaving. Briefly, the optimized method facilitates the determination of silicon content in rice and other plants and could lay a theoretical foundation for further research of the mechanism of silicon absorbance and transportation.