选取微型月季(Rosa hybrida)幼嫩离体叶片(叶龄12天)为外植体, 以MS为基本培养基, 研究不同配比的植物生长调节剂对愈伤组织诱导、不定芽诱导和生根培养的影响。结果表明: (1) 诱导愈伤组织的最佳培养基配比为1.0 mg·L^–16-BA+3.0 mg·L^–12,4-D, 诱导率为100%; (2) 6-BA在微型月季不定芽诱导中起着关键作用, 诱导不定芽产生的植物生长调节剂配比为1.0 mg·L^–16-BA +(0.05–0.5) mg·L^–1NAA+(0.02–0.2) mg·L^–1TDZ, 其中最适配比1.0 mg·L^–16-BA+0.1 mg·L^–1NAA+0.02 mg·L^–1TDZ的愈伤组织再分化率达到92.6%; (3) 生长素对微型月季的生根有重要影响, 以1/4MS+0.1 mg·L^–1NAA为生根培养基可获得100%的生根率。实验最终建立了微型月季组织培养和高频离体再生体系。

We used leaves (12-d-old) of Rosa hybrida as explants and MS medium to examine the effect of combinations and concentrations of growth regulators on callus, adventitious buds and root induction. The optimal medium for inducing calli was MS medium with 3.0 mg·L^–12,4-D+1.0 mg·L^–16-BA; 100% callus induction ratio was obtained. 6-BA played a key role in adventitious bud induction. Adventitious buds were regenerated from calli on MS medium containing 1.0 mg·L^–16-BA, (0.05–0.5) mg·L^–1NAA and (0.02–0.2) mg·L^–1TDZ. The callus redifferentiation ratio was about 92.6% in MS medium supplied with 1.0 mg·L^–16-BA+0.1 mg·L^–1NAA+0.02 mg·L^–1TDZ. Auxin is an important factor for inducing rooting; 100% rooting was obtained on 1/4 MS medium containing 0.1 mg·L^–1NAA. We established a tissue culture and high-frequency regeneration system of R. hybrida.