综合隶属函数法评价花生品种抗旱性与AhNCED1基因表达的关系
收稿日期: 2015-01-09
修回日期: 2015-05-13
网络出版日期: 2015-09-06
基金资助
广东省科技计划项目
Relationship Between Drought Resistance Evaluated by Comprehensive Subordinate Function Values and AhNCED1 Expression in Peanut Varieties
Received date: 2015-01-09
Revised date: 2015-05-13
Online published: 2015-09-06
利用综合隶属函数法评价不同产地8个花生品种幼苗的抗旱能力, 同时分析各品种花生在模拟干旱处理2小时和12小时后叶片AhNCED1基因表达量的变化, 探讨花生品种抗旱性与叶片AhNCED1基因表达的关系, 建立花生(Arachis hypogaea)幼苗时期通过AhNCED1基因表达变化检测花生抗旱性等级的方法。结果显示, 干旱处理2小时, 各品种叶片AhNCED1基因表达量迅速增加, 与处理前相比差异极显著; 干旱处理12小时, 各品种花生叶片AhNCED1基因表达量均降低, 但仍均高于处理前水平。综合隶属函数值定量反映各品种抗旱性强弱顺序为: 花育24号>福花13号>粤油7号>中花15号>中花16号>航花2号>福花9号>北海1号。干旱处理2小时各品种花生叶片AhNCED1表达量变化大小依次为: 福花13号>花育24号>中花16号>中花15号>航花2号>粤油7号>北海1号>福花9号。其中, 抗旱性强的品种为花育24号和福花13号, 抗旱性弱的品种为福花9号和北海1号, 其余4个品种为中等抗旱。该抗旱性评价结果与这些品种在生产上的表现一致, 干旱胁迫2小时花生品种叶片AhNCED1基因的表达量变化与其抗旱性一致。因此, AhNCED1基因表达量可作为苗期花生品种抗旱性等级的量化指标之一。
龙海涛, 李丽梅, 谢泽虹, 刘帅, 李晓云, 邓斌, 刘海燕, 李玲 . 综合隶属函数法评价花生品种抗旱性与AhNCED1基因表达的关系[J]. 植物学报, 2015 , 50(6) : 706 -712 . DOI: 10.11983/CBB15001
We used comprehensive subordinate function values to assess drought resistance of eight representative peanut varieties and simultaneously analyzed the expression of AhNCED1 in peanut leaves under drought stress. We discuss the relation between drought resistance of different peanut varieties and the expression of AhNCED1 with the result of comprehensive subordinate function values. We established a molecular method to qualify the grade of drought resistance of peanut in seedling period by using quantitative PCR to test the expression of AhNCED1 and filter the peanut varieties with drought resistance. AhNCED1 expression increased rapidly after drought treatment for 2 h, which was related to drought resistance of peanuts. After drought treatment for 12 h, the expression decreased and was higher than the control. The comprehensive subordinate function values reflect the resistance order of different peanuts: Huayu 24>Fuhua 13>Yueyou 7>Zhonghua 15>Zhonghua 16>Hanghua 2>Fuhua 9>Beihai 1. AhNCED1 expression after water stress treatment for 2 h reflects the resistance order: Fuhua 13>Huayu 24>Zhonghua 16>Zhonghua 15>Hanghua 2>Yueyou 7>Beihai 1>Fuhua 9. Fuhua 9 and Beihai 1 are weaker in drought resistance and Huayu 24 and Fuhua 13 are stronger. As well, under drought stress, peanut drought resistance is positively correlated with the expression of Ah-NCED1 in leaves. We can filter peanut varieties with stronger drought resistance by testing the expression of AhNCED1.
陈艳萍, 何金丽, 李丽梅, 李玲（2013）. 不同花生品种抗旱生理与AhNCED1基因表达的关系. 植物生理学报 49,369-374.
程曦,王月福, 赵长星,王铭伦,康玉洁,宋伟（2010）. 不同生育期干旱对花生衰老特性及产量的影响. 花生学报 39, 33-36.
段志龙, 王长军(2010). 作物抗旱性鉴定指标及方法. 中国种业 9, 19-22.
刘吉升,李玲(2006). 不同品种花生的抗旱能力及其内源ABA的关系. 植物生理学通讯 42,1115-1116.
刘江娜, 邓晓艳, 李志博, 刘菲菲, 章杰, 郗忠玲, 魏亦农(2010). 棉花9-顺式环氧类胡萝卜素双加氧酶基因在干旱条件下的表达分析. 石河子大学学报（自然科学版）28,546-550.
万小荣, 莫爱琼, 郭小建, 杨妙贤, 余土元, 曹锦萍(2010). 含异位表达花生基因的拟南芥提高耐渗透胁迫能力. 作物学报 36,1440-1449.
王贺正,马均,李旭毅(2007). 水分胁迫对水稻结实期活性氧产生和保护系统的影响. 中国农业科学 40,1379-1386.
吴影,宋丰顺,陆徐忠,赵伟,杨剑波,李莉(2007). 实时荧光PCR技术定量检测转基因大豆方法的研究. 作物学报 10,1733-1737.
张殿忠, 汪沛洪, 赵会贤(1990). 测定小麦叶片游离脯氨酸含量的方法. 植物生理学通讯 26 (4),62-65.
张智猛,万淑波,戴良香,宋文武,陈静,石运庆(2011). 花生抗旱性指标的筛选与评价. 植物生态学报, 35(1), 100-109.
Bo Hu, Lan Hong , Xu Liu, Suni Xiao, Yan Lv, Ling Li(2013a).The higher expression and distribution of 9-cis-epoxycarotenoid dioxygenase1 (AhNCED1) from Arachis hyopgaea L. contribute to tolerance to water stress in a drought-tolerant cultivar. Acta Physiologiae Plantarum 35,1667-1674.
Bo Hu, Lan Hong , Xu Liu, Su-Ni Xiao, Yan Lu, Ling Li(2013b). Identification of different ABA biosynthesis sites at seedling and fruiting stages in Arachis hypogaea L. following water stress. Plant Growth Regulaton 70,131-140.
Fukao T, Yeung E, Bailey-Serres J (2011). The submergence tolerance regulator SUB1A mediates crosstalk between submergence and drought tolerance in rice. Plant Cell 23, 412-427.
Liang JH, Yang LX, Chen X, Li L, Guo DL, Li HY, Zhang BY(2009). Cloning and characterization of the promoter of the 9-cis-epoxycarotenoid dioxygenase gene in Arachis hypogaea L. Biosci Biotech Bioch 73,2103-2106.
Liu X, Hong L, Li XY, Yao Y, Hu B, Li L (2011). Improved drought and salt tolerance in transgenic Arabidopsis overexpressing a NAC transcriptional factor from Arachis hypogaea. Biosci Biotech Bioch, 75: 100614-100618.
Su LC, Xu L, Chen YP, Li L (2012). Isolation of AhDHNs from Arachishypogaea L. and evaluation of AhDHNs expression under exogenous abscisic acid (ABA) and water stress. Afr J Biotechnol 11, 11221-11229.
Wan XR, Li L(2006). Regulation of abscisic acid level and drought tolerance of transgenic Arabidopsis by ectopic expression of a peanut 9-cis-epoxycarotenoid dioxygenase gene which is in response to water stress, high salinity and exogenous ABA. Biochem Biophy Res Commun 347,1030-1038.
Yanping Chen, Ling Li , Pengwei Hu, Jinli He, Zhiwei Ru, Bo Hu，Liangchen Su(2014). Relationship between drought resistance and AhNCED1 expression in peanut varieties from four provinces in China. Journal of Food, Agriculture & Environment 12,509-514.
Yue Y, Zhang MC, Zhang JZ, Tian XL, Duan LS, Li ZH (2012). Overexpression of the AtLOS5 gene increased abscisic acid level and drought tolerance in transgenic cotton. J Exp Bot 63,3741-3748.
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