黄金树B类MADS-box基因表达特征分析
收稿日期: 2015-03-12
修回日期: 2015-07-05
网络出版日期: 2016-03-31
基金资助
十二五国家科技支撑项目(No.2012BAD21B03)
Expression Analysis of B-class MADS-box Genes from Catalpa speciosa
Received date: 2015-03-12
Revised date: 2015-07-05
Online published: 2016-03-31
采用同源克隆技术, 从黄金树(Catalpa speciosa)花芽中克隆得到B类MADS-box基因CaspAP3和CaspPI的cDNA序列。序列分析表明, CaspAP3基因cDNA序列的完整开放阅读框(ORF)为696 bp, 编码231个氨基酸残基; CaspPI基因cDNA序列的ORF为639 bp, 编码212个氨基酸残基。蛋白质序列相似性比对和分子系统发生分析表明, CaspAP3属于AP3/DEF进化支, 其C末端包含保守的euAP3基序和PI-derived基序, 而CaspPI聚类于PI/GLO进化支, 其C末端包含保守的PI基序。半定量RT-PCR分析结果表明, CaspAP3和CaspPI基因均仅在花瓣和雄蕊中表达。实时荧光定量PCR分析表明, CaspAP3和CaspPI基因在花瓣和雄蕊原基分化期至成熟期均有表达, 这2个基因在雄蕊中表达高峰出现的时间均早于花瓣; 且花瓣中的CaspAP3和CaspPI基因表达高峰均出现在快速伸长阶段; 这与花瓣和雄蕊的形态发育阶段相吻合。
景丹龙, 夏燕, 张守攻, 王军辉 . 黄金树B类MADS-box基因表达特征分析[J]. 植物学报, 2016 , 51(2) : 210 -217 . DOI: 10.11983/CBB15053
The cDNA sequence of B-class MADS-box genes CaspAP3 and CaspPI involved in flower development were isolated from the flower bud of Catalpa speciosa by homology cloning. The open reading frame (ORF) of CaspAP3 was 696 bp, encoding 231 amino acids, and the ORF of CaspPI was 639 bp, encoding 212 amino acids. Molecular phylogeny analysis and protein sequence alignment suggested that CaspAP3 groups with the AP3/DEF lineages and the C domain of CaspAP3 contained two conserved motifs, euAP3 and PI-derived motifs. However, CaspPI grouped with the PI/GLO lineages, and the C domain of CaspPI contained a conserved PI motif. CaspAP3 and CaspPI mRNA expression was mainly concentrated in petals and stamens but occurred at the primordium to mature stages of petals and stamens during morphological differentiation of organs. The expression of both factors peaked earlier in stamens than in petals, and the peak expression for both in petals appeared at the stage of rapid elongation. Meanwhile, the time of expression was the same with morphological differentiation of petals and stamens.
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