红厚壳(Calophyllum inophyllum)为藤黄科红厚壳属多年生木本植物, 有很高的药用价值。该研究以红厚壳带节茎段为外植体, 探讨生长调节剂对腋芽萌发及丛生芽诱导、伸长和试管苗生根的影响。研究结果表明, 外植体腋芽萌发和丛生芽诱导效果最好的培养基是MS+NAA1.0+TDZ0.5, 在此条件下培养21天后, 转入添加0.5 g·L–1活性炭且无生长调节剂的MS培养基, 可有效促进不定芽的伸长。将带不定芽的外植体先在附加1.0 mg·L–1NAA的1/2MS培养基上进行生根诱导4周, 之后转入附加1.0 g·L–1活性炭的无激素培养基进行根的伸长培养, 这样的两步生根法能有效促进红厚壳生根。
Calophyllum inophyllum is an important medicinal tree belonging to the Guttiferae family. We used stem segments with a node as explants for bud propagation and plantlet regeneration and examined the effects of plant growth regulators on axillary bud germination, propagation, bud elongation and rooting from adventitious buds. The suitable medium for axillary bud germination and propagation was MS supplemented with NAA1.0 and TDZ0.5. Culture of explants on this medium for 21 days and then transfer to hormone-free medium supplied with 0.5 g·L–1 active carbon promoted the elongation of adventitious buds. We developed a two-step method for rooting. The explants with adventitious buds were first cultured on 1/2 MS medium with 1 mg·L–1NAA for 4 weeks, then on hormone-free medium supplied with 1.0 g·L–1 active carbon. This two-step rooting method accelerated rooting efficiently.
梅文莉，曾艳波，戴好富，郑学勤（2006）．红厚壳挥发油化学成分．植物资源与环境学报 15，74-75．
纳智（2005）．滇南红厚壳种子油的脂肪酸成分．热带亚热带植物学报13，505-506．
陶忠良, 方佳, 赖齐贤（2003）．红厚壳在海南岛的分布及其生长习性．资源开发与市场 19,85-87．
阳辛凤，方佳，陶忠良（2001）．海棠果的开发应用价值分析．中国野生植物资源20，33-35．
张存旭，宋敏，赵忠等（2004）．栓皮栎茎段离体培养的研究．西北植物学报24，1260-1265.
Ahmad N, Anis M (2007). Rapid clonal multiplication of a woody tree Vitex negumdo L. through axillary shoots proliferation. Agroforest Syst 71, 195-200 .
Cechinel Filho V, Meyre-Silva C, Niero R (2009).Chemical and pharmacological aspects of the genus Calophyllum. Chem Biodivers 6(3):313-27.
Dai HY ,Zhang ZH ,Guo XW (2007).Adventitious bud regeneration from leaf and cytoledon explants of Chinese hawthorn (Crataegus pinnatifida Bge .Var .major N .E .Br) . In Vitro Cell Dev Biol Plant 43, 2-8 .
Dhavala A ,Rathore TS (2010).Micropropagation of Embelia ribes Burm f. through proliferation of adult plant axillary shoots. In Vitro Cell Dev Biol Plant 46,180-191.
Itoigawa M, Ito C, Tan HT, Kuchide M, Tokuda H, Nishino H, Furukawa H(2001). Cancer chemopreventive agents, 4-phenylcoumarins from Calophyllum inophyllum. Cancer Lett 169,15-19.
Husain MK ,Anis M Shahzad A (2007). In vitro propagation of Indian Kino (Pterocarpus marsupium Roxb.)using Thidiazuron. In Vitro Cell Dev Biol Plant 43, 59-64.
Malarvizhi P, Ramakrishnan N (2011). GC-MS analysis of biologically active compounds in Leaves of Calophyllum inophyllum L. Int J ChemTech Res 3,806-809
Nair LG, Seeni S（2003）. In vitro multiplication of Calophyllum apetalum (Clusiaceae), an endemic medicinal tree of the Western Ghats. Plant Cell Tissue Organ Cult 75,169-174 .
Pawar KD, Joshi SP, Thengane SP (2011).Association between chemical and genetic variation in Calophyllum inophyllum, a medicinally important tree of the Western Ghats of India.Plant Syst Evol 292:257-265.
Rajeswari V, Paliwal K(2006). In vitro propagation of Albizia odoratissima L.F.(Benth.) from cotyledonary node and leaf nodal explants. In Vitro Cell Dev Biol Plant 42, 399-404.
Siddique I, Anis M (2009). Direct plant regeneration from nodal explants of Balanites aegyptiaca L.(Del.):a valuable medicinal. New Forests 37, 53-62.
Tsai SC, Liang YH, Chiang JH, Liu FC, Lin WH, Chang SJ, Lin WY, Wu CH, Weng JR (2012).Anti-inflammatory effects of Calophyllum inophyllum L. in RAW264.7 cells. Oncol Rep 28,1096-102
Vengadesan G ,Pijut PM (2009) .In vitro propagation of northern red oak (Qvercus rubra L.) . In Vitro Cell Dev Biol Plan 45,474-482.
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