9-羰基-10,11-去氢泽兰酮为紫茎泽兰(Eupatorium adenophorum)的主要致肝脏毒性成分及杀虫的生物活性成分。从紫茎泽兰叶片中分离提纯得到9-羰基-10,11-去氢泽兰酮(Euptox A)标准品, 建立了高效液相色谱法测定紫茎泽兰中Euptox A含量的分析方法。采用C18反相色谱柱, 柱温30°C, 以甲醇-水(60:40, v/v)为流动相、流速为0.8 mL·min–1、检测波长为255 nm进行测定。Euptox A在紫茎泽兰中的添加回收率为97.3%–103.7%, 检测限为0.4 μg·g–1。利用建立的方法测定Euptox A
在紫茎泽兰体内分布与积累的动态变化规律。结果表明, Euptox A主要分布在紫茎泽兰的叶片中, 且在营养生长期积累量高, 生殖生长期积累量低。该方法快速、简捷, 可用于紫茎泽兰原料及其产品中Euptox A成分的测定。
We isolated and purified the component of 9-oxo-10,11-dehydroagerophorone (Euptox A), a hepatotoxin and an insecticide constituent, from Eupatorium adenophorum leaves by silica gel column chromatography and reverse-phase high performance liquid chromatography (HPLC). We developed an HPLC method for determining Euptox A from E. adenophorum, which was confirmed by HPLC-DAD-MS. The chromatographic separation was achieved on a Cosmosil Rp C18 column with methanol:water (60:40, v/v) as the mobile phase, flow rate 0.8 mL·min–1 and wavelength 255 nm. Data were corroborated by HPLC-MS with electro-spray ionization of positive ion mode. The limit of detection for Euptox A was 0.4 μg·g–1. The recoveries were 97.3%–103.7% and relative standard deviations 2.4%–3.5%. Euptox A was mainly distributed in leaves (1.34–8.41 mg·g–1) and only a small amount was detected in flowers, stems, or roots (< 0.06 mg·g–1). Euptox A mainly accumulated in plant leaves in the vegetative period (from May to October), with less accumulation in the
reproductive period (from November to the next April). The established HPLC method is simple, quick and could be used for component determination in E. adenophorum products for quality control and safety evaluation.
曹坳程，芮昌辉，郭美霞，袁会珠，杨代斌，陈福良（2006）. 紫茎泽兰的化学防除及生态修复效果安全与环境学报，，6（3）：80~85
周自玮，段新慧，徐驰，奎嘉祥（2006）. 紫茎泽兰饲喂山羊试验研究,饲料工业27（19）： 25~28
Bohlman F, Gupta P K(1981). Six cadinane derivatives from Ageratina adenophora. Phytochemistry, 20:1432~1433
He L, Hou J, Gan M L, SHi JG, Chantrapromma S, Fun HK, Williams L D, SUNG H Y (2008). Journal of Natural Products, 71:1485-1488
He L, Yang J, Cao AC，Liu YM， An Y, Shi JG(2006). A new Sequiterpenoid from Eupatorium adenophorum Spreng. Chinese Journal of Chemistry. 24, 1375-1377
Kaushal V, Dawra R K, Sharma O P, Kurade N P(2001). Biochemical alterations in the blood plasma of rats associated with hepatotoxicity induced by Eupatorium adenophorum.Veterinary Research Communications, 2001, 25: 601~608 Bohlman F, Gupta P K. Phytochemistry， 20：1432-1433
Oelrichs PB, Calanasan CA, Madleod J K, Seawright AA, Ng JC(1995). Isolation of a compound from Eupatorium adenophorum (spreng.) [Ageratina adenophora (spreng.)] causing hepototoxicity in mice.Natural Toxins. 3: 350~354
Sun XY, Lu ZH, Sang WG(2004). Review on studies of Eupatorium adenophorum-an important invasive species in China.Journal of Forestry Research, 15:319~322
Verma A, Yadava BPS, Sampath KT. Ind. J（1987）.Possible use of Eupatorium adenophorum Spreng. in animal feeding. Anim. Nutr. , 4:189~192
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