创刊30年特邀综述

我国观赏花卉品质形成的功能基因研究进展

展开
  • 华南师范大学生命科学学院, 广东省植物发育生物工程重点实验室, 广州 510631

收稿日期: 2013-08-19

  修回日期: 2013-09-11

  网络出版日期: 2013-09-26

Progress in the Functional Research of Flower-quality-related Genes in Ornamental Flowers in China

Expand
  • Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, College of Life Sciences, South China Normal University, Guangzhou 510631, China

Received date: 2013-08-19

  Revised date: 2013-09-11

  Online published: 2013-09-26

摘要

花色、花香、花形和花期等品质性状与花卉商品价值体现有着直接的关系, 花卉品质形成相关的功能基因研究十分重要。该文综述了近10年来我国观赏花卉品质形成相关的功能基因研究成果, 以期为花卉分子育种研究提供理论参考。

本文引用格式

王小菁, 杨玉萍 . 我国观赏花卉品质形成的功能基因研究进展[J]. 植物学报, 2013 , 48(5) : 471 -480 . DOI: 10.3724/SP.J.1259.2013.00471

Abstract

The characters of flower quality such as flower color, scent, shape, and blooming time are related directly to the commodity value of the plant. Functional research in flower-quality-related genes of ornamental flowers is important. We review the achievements in this area over the past 10 years in China to provide information for molecular design breeding.

参考文献

[1]安利忻,刘荣维,陈章良,李毅.花分生组织决定基因AP1转化矮牵牛的研究[J][J].植物学报, 2001, 43

(1):63-66
[2]白凌,马月萍,戴思兰.烟草转DFL基因植株的鉴定[J][J].分子植物育种, 2007, 5

(3):329-334
[3]蔡蕾,张晓红,沈红香,高俊平.乙烯对不同切花月季品种开花和衰老的影响[J][J].园艺学报, 2002, 29

(5):467-472
[4]陈段芬.中国水仙花形、花色发育基因(NTMADS1、NtMADS3、NTPDS1和NTPZDS1)的克隆和转化[D]. 北京:中国林业科学院. pp. 65–67.., 2008,

:-
[5]陈璟,李名扬,闫明旭,郭余龙 .矮牵牛PMADS9基因的结构特征和mRNA的表达分析[J][J].园艺学报, 2011, 38

(1):108-116
[6]傅永福,赵德刚,韩玉珍,于静娟,孟繁静 .花同源异型基因FBP2调节叶片中过氧化物酶的表达[J][J].植物生理学报, 2000, 26

(4):293-296
[7]韩科厅,赵莉,唐杏姣,胡可,戴思兰 .菊花花青素苷合成关键基因表达与花色表型的关系[J][J].园艺学报, 2012, 39

(3):516-524
[8]洪艳,白新祥,孙卫,贾锋炜,戴思兰.菊花品种花色表型数量分类研究[J][J].园艺学报, 2012, 39

(7):1330-1340
[9]胡可,韩科厅,戴思兰.环境因子调控植物花青素苷合成及呈色的机理[J][J].植物学报, 2010, 45

(3):307-317
[10]黄志刚,梁敏婷,邹德乐,高苏娟,王小菁.外源NH4+对非洲菊舌状花着色与生长的影响[J][J].园艺学报, 2009, 36

(4):599-604
[11]姜丹,梁建丽,陈晓丽,洪波,贾文锁,赵梁军.拟南芥花期基因FT转化切花菊‘神马’[J][J].园艺学报, 2010, 37

(3):441-448
[12]李红梅,丁岳炼,林燕飞,陈伟文,黄新敏,何生根 .麝香百合液泡膜水孔蛋白基因LlTIP2的克隆及生物信息学分析[J][J].热带作物学报, 2011, 32

(10):1868-1872
[13]李静,车代弟.花卉基因工程育种研究进展[J][J].分子植物育种, 2004, 2

(1):1010-1104
[14]李美茹,陈金婷,孙梓健,陈贻竹,李洪清 .花卉分子育种的研究进展[J][J].热带亚热带植物学报, 2003, 11

(1):87-92
[15]李瑞红,范燕萍.白姜花不同开花时期的香味组分及其变化[J][J].植物生理学通讯, 2007, 43

(1):176-180
[16]林燕飞,李红梅,丁岳练,黄新敏,冼锡金,何生根 .唐菖蒲质膜水孔蛋白基因GhPIP1; 1 的克隆及表达分析[J][J].园艺学报, 2013, 40

(1):145-154
[17]马男,蔡蕾,陆旺金,谭辉,高俊平.外源乙烯对月季(Rosa hybrida)切花花朵开放的影响与乙烯生物合成相关基因表达的关联[J][J].中国科学辑生命科学, 2005, 35

(2):104-114
[18]孟祥春,彭建宗,王小菁.光和糖对非洲菊花色素苷积累及CHS、DFR基因表达的影响[J][J].园艺学报, 2007, 34

(1):227-230
[19]沈红香,刘晓辉,蔡蕾,高俊平 .三类切花月季品种花器官乙烯生成量和ACC含量时空变化动态[J][J].中国农业科学, 2004, 37

(12):1899-1903
[20]孙崇波,向林,李小白,秦德辉,李伯钧,郭方其,吴超.蕙兰Flowering locus T基因的克隆及其对开花的影响[J][J].中国农业科学, 2013, 46

(7):1419-1425
[21]孙卫,李崇晖,王亮生,戴思兰.菊花不同花色品种中花青素苷代谢分析[J][J].植物学报, 2010, 45

(3):327-336
[22]孙翊 .瓜叶菊PCFH基因对花青素苷合成及花色的影响[D]. 博士论文. 北京:北京林业大学. pp. 60–63 .., 2011,

:-
[23]王海萍,晏慧君,张颢,蹇洪英,王其刚,邱显钦,李淑斌,周宁宁,唐开学 .月季(Rosa chinensis)丁香酚合成酶基因RcEGS1的克隆及其表达分析[J][J].园艺学报, 2012, 39

(7):1387-1394
[24]王小菁,孟祥春,彭建宗 .花色形成与花生长的调控[J][J].西北植物学报, 2003, 23

(7):1105-1110
[25]于静,董丽丽,郗琳,赵瑞艳,马男,赵梁军.切花菊‘神马’细胞分裂素合成酶基因DgIPT3参与侧枝发育的功能分析[J]. 721–728.[J].园艺学报, 2012, 39

(4):721-728
[26]余义勋,包满珠 .反义ACO基因导入获得瓶插寿命长的香石竹植株[J][J].园艺学报, 2004, 31

(3):633-636
[27]余义勋,包满珠 .通过转重复结构的ACC氧化酶基因延长香石竹的瓶插期[J][J].植物生理与分子生物学学报, 2004, 30

(5):541-545
[28]赵昶灵,郭维明,陈俊愉 .植物花色形成及其调控机理[J][J].植物学通报, 2005, 22

(1):70-81
[29]郑鸿昌 .夜来香均一化全长cDNA文库的构建与花香相关基因的遗传转化[D]. 福建:福建农业大学. pp.29–40.., 2012,

:-
[30]郑亚东,郭余龙,陈旭,李艳冬,欧建龙,李名扬 .GhMADS3基因组成型表达对矮牵牛花形和花色的影响[J][J].园艺学报, 2007, 34

(4):985-990
[31]朱高潽.山茶花MADS-box家族B类基因克隆及在重瓣花形成中的作用[D]. 北京:中国林业科学院. pp. 62–77.., 2011,

:-
[32]A′lvarez-Buylla ER, Ambrose BA, Flores-Sandoval E, Englund M, Garay-Arroyo A, Garc′a-Ponce B, de la Torre-Ba′rcena E, Espinosa-Mat′as S, Mart′nez E, Pin′eyro-Nelson A, Engstro¨m P, Meyerowitz EM .B-Function Expression in the Flower Center Underlies the Homeotic Phenotype of Lacandonia schismatica (Triuridaceae)[J][J].Plant Cell, 2010, 22

(11):3543-3559.
[33]An FM, Hsiao SR, Chan MT.Sequencing-based approaches reveal low ambient temperature-responsive and tissue-specific microRNAs in Phalaenopsis orchid[J][J]., 2011, 6

(5):-
[34]Catherine R, Feng X, Hu X, Luo D.Genetic control of floral zygomorphy in pea (Pisum sativumL.)[J][J].Proc Natl Acad Sci USA, 2008, 105

(30):10414-10419
[35]Chang YY, Chu YW, Chen CW, Leu WM, Hsu HF, Yang CH.Characterization of Oncidium ‘Gower Ramsey’ transcriptomes using 454 GS-FLX pyrosequencing and their application to the identification of genes associated with flowering time[J][J].Plant Cell Physiol, 2011, 52

(9):1532-1545
[36]Chang YY, Kao NH, Li JY, Hsu WH, Liang YL, Wu JW, Yang CH .Characterization of the possible roles for B class MADS box genes in regulation of perianth formation in orchid[J][J].Plant Physiol, 2010, 152

(2): 837-853
[37]Chen D, Guo B, Hexige S, Zhang T, Shen D, Ming F .SQUA -like genes in the orchid Phalaenopsis are expressed in both vegetative and reproductive tissues[J][J].Planta, 2006, 226

(2): 369-380
[38]Chen SY, Tsai HC, Raghu R, Do YY, Huang PL.cDNA cloning and functional characterization of ETHYLENE INSENSITIVE 3 orthologs from Oncidium Gower Ramsey involved in flower cutting and pollinia cap dislodgement[J][J].Plant Physiol Biochem, -, 2011, 49

(10):1209-1219
[39]Chen X, Zhou X, Xi L, Li J, Zhao R, Ma N, Zhao L.Roles of DgBRC1 in regulation of lateral branching in Crysanthemum (Dendranthema×grandiflora cv.Jinba)[J][J]., 2013, 8

(4):e61717-
[40]Chiou CY, Pan HA, Chuang YN, Yeh KW.Differential expression of carotenoid-related genes determines iversified carotenoid coloration in floral tissues of Oncidium cultivars. [J].Planta, 2010, 232

(4):937-948
[41]Chiou CY, Wu K, Yeh KW.Characterization and promoter activity of chromoplast specific carotenoid associated gene (CHRC) from Oncidium Gower Ramsey[J][J].Biotech Lett, 2008, 30

(10):1861-1866
[42]Chiou CY, Yeh KW.Differential expression of MYB gene (OgMYB1) determines color patterning in floral tissue of Oncidium Gower Ramsey. [J].Plant Mol Biol, 2008, 66

(4):379-388
[43]Chou ML, Shih MC, Chan MT, Liao SY, Hsu CT, Haung YT, Chen JJ, Liao DC, Wu FH, Lin CS .Global transcriptome analysis and identification of a CONSTANS-like gene family in the orchid Erycina pusilla. [J].Planta, 2013, 237

(6):1425-1441
[44]Dai F, Zhang C, Jiang X, Kang M, Yin X, Lu P, Zhang X, Zheng Y, Gao J.RhNAC2 and RhEXPA4 are involved in the regulation of dehydration tolerance during the expansion of rose petals.[J].Plant Physiol, 2012, 160

(4):2064-2082
[45]Dudareva N, Klempien A, Mublemann JK, Kaplan I .Biosynthesis, function and metabolic engineering of plant volatile organic compounds.[J].New Phytol, 2013, 198

(1):16-32
[46]Dudareva N, Cseke L., Blanc V.M., Pichersky E. Evolution of floral scent in Clarkia: novel patterns of S-linalool synthase gene expression in the C. breweri flower.[J].Plant Cell, 1996, 8

(7):1137-1148
[47]Feng X, Zhao Z, Tian Z, Xu S, Luo Y, Cai Z, Wang Y, Yang J, Wang Z, Weng L, Chen J, Zheng L, Guo X, Luo J, Sato S, Tabata S, Ma W, Cao X, Hu X, Sun C, Luo D.Control of petal shape and floral zygomorphy in Lotus japonicus. [J].Proc Natl Acad Sci, 2006, 103

(13):4970-4975
[48]Fu Z, Wang H, Liu J, Liu J, Wang J, Zhang Z, Yu Y .Cloning and characterization of a DCEIN2 gene responsive to ethylene and sucrose in cut flower carnation[J][J]., 2011, 105

(3):447-455
[49]Fu Z, Wang H, Liu J, Zeng H, Zhang J, Kuang X, Yu Y .Molecular cloning and characterization of carnation EBF1 gene during flower senescence and upon ethylene exposure and sugar[J][J].Agricul Sci China, 2011, 10

(12):1872-1880
[50]Guo B, Zhang T, Shi J, Chen D, Shen D, Ming F.Cloning and characterization of a novel PI-like MADS-box gene in Phalaenopsis orchid[J][J].DNA Seq, 2008, 19

(3):332-339
[51]Hsiao YY, Huang TH, Fu CH, Huang SC, Chen YJ, Huang YM, Chen WH, Tsai WC, Chen HH.Transcriptomic analysis of floral organs from Phalaenopsis orchid by using oligonucleotide microarray[J][J].Gene, 2013, 518

(1):91-100
[52]Hsiao YY, Pan ZJ, Hsu CC, Yang YP, Hsu YC, Chuang YC, Shih HH, Chen WH, Tsai WC, Chen HH.Research on orchid biology and biotechnology[J][J].Plant Cell Physiol, 2011, 52

(9):1467-1486
[53]Huang W, Fang Z, Zeng S, Zhang J, Wu K, Chen Z, Teixeira da Silva JA, Duan J .Molecular cloning and functional analysis of three FLOWERING LOCUS T (FT) homologous genes from Chinese Cymbidium[J][J].Int J Mol Sci, 2012, 13

(9):11385-11398
[54]Huang Z, Liang M, Peng J, Xing T, Wang X.Exogenous ammonium inhibits petal pigmentation and expansion in Gerbera hybrida[J][J].Physiol Plant ., 2008, 133

(2):254-265
[55]Irish VF.The Arabidopsis petal: a model for plant organogenesis. [J].Trends Plant Sci, 2008, 13

(8):430-436
[56]Irish VF.The flowering of Arabidopsis flower development[J].Plant J, 2010, 61

:1014-1028
[57]Jack T, Brockman LL, Meyerowitz EM.The homeotic gene APETALA3 of Arabidopsis thaliana encodes a MADS box and is ex-pressed in petalsand stamens[J][J].Cell, 1992, 68

(4):683-689
[58]Knudsen JT, Eriksson R, Gershenzon J, Stahl B.Diversity and distribution of floral scent[J][J]., 2006, 72

:-
[59]Krizek BA, Fletcher JC.Molecular mechanisms of flower development: an armchair guide[J][J].Nat Rev Genet, 2005, 6

(9):688-698
[60]Kuang Q, Li L, Peng J, Sun S, Wang X .Transcriptome analysis of Gerbera hybrida ray florets: putative genes associated with gibberellin metabolism and signal transduction[J][J]., 2012, 8

:-
[61]Lan J, Yu R, Yu Y, Fan Y .Molecular cloning and expression of Hedychium coronarium farnesyl pyrophosphate synthase gene and its possible involvement in the biosynthesis offloral and wounding/herbivory induced leaf volatile sesquiterpenoids[J][J].Gene, 2013, 518

(2):360-367
[62]Lee J, Lee I .Regulation and function of SOC1, a flowering pathway integrator[J][J].J Exp Bot, 2010, 61

:2247-2254
[63]Li R, Fan Y .Molecular cloning and expression analysis of a terpene synthase gene, HcTPS2, in Hedychium coronarium[J][J].Plant Mol Biol Rep, 2011, 29

(1):35-42
[64]Li R, Wang A, Sun S, Liang S, Wang X, Ye Q, Li H .Functional characterization of FT and MFT ortholog genes in orchid (Dendrobium nobile Lindl) that regulate the vegetative to reproductive transition in Arabidopsis[J][J].Plant Cell Tiss Organ Cult, 2012, 111

:143-151
[65]Li Y, Wu Z, Ma N, Gao J.Regulation of the rose Rh-PIP2:1promoter and abiotic stresses in Arabidopsis[J] [J].Plant Cell Rep, 2009, 28

(2):185-196
[66]Liang JL, Zhao LJ, Challis R, Leyse O .Strigolactone regulation of shoot branching in chrysanthemum (Dendranthema×grandiflorum)[J][J].J Exp Bot, 2010, 61

(11):3069-3078
[67]Liang S, Ye Q, Li R, Leng J, Li M, Wang X, Li H .Transcriptional regulations on the low-temperature –induced floral transition in an Orchidaceae species, dendrobium nobile:An expressed sequence tags analysis[J][J]., 2012,

:-
[68]Liu J, Li J, Wang H, Fu Z, Liu J, Yu Y .Identification and expression analysis of ERF transcription factor genes in petunia during flower senescence and in response to hormone treatments[J][J].J Exp Bot, 2011, 62

(2):825-840
[69]Liu Q, Xu K, Ma N, Zeng L, Zhao L .Isolation and functional characterization of DgZFP: a gene encoding a Cys2/His2-type zinc finger protein in chrysanthemum[J][J].Mol Biol Rep, 2010, 37

(2):1137-1142
[70]Liu XJ, Chuang YN, Chiou CY, Chin DC, Shen FQ, Yeh KW .Methylation effect on chalcone synthase gene expression determines anthocyanin pigmentation in floral tissues of two Oncidium orchid cultivars[J][J].Planta, 2012, 236

(2):401-409
[71]Ma N, Tan H, Liu X, Xue J, Li Y, Gao J.Transcriptional regulation of ethylene receptor and CTPs genes involved in ethylene inducud flower full opening in cut rose (rasa hybrida)cv. Samantha[J][J].J Exp Bot, 2006, 57

(11):2763-2773
[72]Ma N, Xue J, Li Y, Liu X, Dai F, Jia W, Luo Y, Gao J .Rh-PIP2:1, a Rose aquaporin gene, is involved in ethylene-regulated petal expansion[J][J].Plant Physiol, 2008, 148

(2):894-907
[73]Meng X, Xing T, Wang X .The role of light in the regulation of anthocyanin accumulation in Gerbera hybrida[J][J].Plant Growth Regulation, 2004a, 44

:243-250
[74]Meng X, Xing T, Wang X.Regulation of flower development and anthocyanin accumulation in Gerbera hybrida[J][J].J Hort Sci Biotech, 2004b, 79

(2):131-137
[75]Peng J, Lai L, Wang X.PRGL: A cell wall proline-rich protein containning GASA domain in Gerbera hybrida[J][J].Sci China Ser C:Life Sci, 2008, 51

(6):520-525
[76]Peng JZ, Lai LJ, Wang XJ .Temporal and spatial expression analysis of PRGL in Gerbera hybrida[J][J].Mol Biol Rep, 2010, 37

(7):3311-3317
[77]Petroni K, Tonelli C .Recent advances on the regulation of anthocyanin synthesis in reproductive organs[J][J].Plant Sci, 2011, 181

(3):219-229
[78]Powell AE, Lenhard M .Control of organ size in plants[J][J].Curr Biol, 2012, 22

(9):360-367
[79]Su CL, Chao YT, Alex Chang YC, Chen WC, Chen CY, Lee AY, Hwa KT, Shih MC .De novo assembly of expressed transcripts and global analysis of the Phalaenopsis aphrodite transcriptome[J][J].Plant Cell Physiol, 2013, 52

(9):1501-1514
[80]Sun Y, Huang H, Meng L, Hu K, Dai S.Isolation and functional analysis of a homolog flavonoid 3’5’-hydroxylase gene from Pericallis × hybrida[J][J]., 2013,

:-
[81]Tan H, Liu X, Ma N, Xue J, Lu W, Bai J, Gao J.Ethylene-influenced flower opening and expression of genes encoding ETR, CTR and ELN3s in two cut rose cultivars[J][J].Postharvest Biol Technol, 2006, 40

(5):97-105
[82]Taoka K, Ohki I, Tsuji H, Kojima C, Shimamoto K.Structure and function of florigen and the receptor complex[J][J].Trends Plant Sci, 2013, 18

(5):287-294
[83]Varaud E, Brioudes F, Szécsi J, Leroux J, Brown S, Perrot-Rechenmann C, Bendahmane M .AUXIN RESPONSE FACTOR8 regulates Arabidopsis petal growth by interacting with the bHLH transcription factor BIGPETALp[J][J].Plant Cell, 2011, 23

(3):973-983
[84]Wang J, Wang Y, Luo D.LjCYC genes constitute floral dorsoventral asymmetry in Lotus japonicus[J][J].J Integr Plant Biol, 2010, 52

(11):959-970
[85]Wang Z, Chen J, Weng L, Li X, Cao X, Hu X, Luo D, Yang J .Multiple components are integrated to determine leaf complexity in Lotus japonicus[J][J].J Integr Plant Biol, 2013, 55

(5):419-433
[86]Xiang L, Li X, Qin D, Guo F, Wu C, Miao L, Sun C .Functional analysis of FLOWERING LOCUS T orthologs from spring orchid (Cymbidium goeringii Rchb. f.) that regulates the vegetative to reproductive transition[J][J].Plant Physiol Biochem, 2012, 58

:98-105
[87]Xiang L, Zhao K, Chen L.Molecular cloning and expression of Chimonanthus praecox farnesyl pyrophosphate synthase gene and its possible involvement in the biosynthesis of floral volatile sesquiterpenoids[J][J].Plant Physiol Biochem, 2010, 48

(10):845-850
[88]Xu B, Li Z, Zhu Y, Wang H, Ma H, Dong A, Huang H.Arabidopsis genes AS1, AS2 and JAG negatively regulate boundary-specifying genes to promote sepal and petal development[J][J].Plant Physiol, 2008, 146

(2):566-575
[89]Xu S, Luo Y, Cai Z, Cao X, Hu X, Yang J, Luo D .Functional diversity of CYCLEA-like TCP genes in the control of zygomorphic flower development in Lotus japonicus[J][J].J Integr Plant Biol, 2013, 55

(3):221-231
[90]Xue J, Li Y, Tan H, Yang P, Ma N, Gao J .Expression of ethylene biosynthetic and receptor genes in rose floral tissues during ethylene-enhanced flower opening[J][J].J Exp Bot, 2008, 59

(8):2161-2169
[91]Xue J, Yang F, Gao J.Isolation of Rh-PIP1:1, an aquaporin gene and its expression in rose flowers in response to ethylene and water deficit. [J].Postharvest Biol Technol, 2009, 51

:407-413
[92]Yan H,Zhang H,Wang Q,Jian H,Qiu X,Tang K.Isolation and identification of a putative scent-related gene RhMYB1 from rose[J][J].Mol Biol Rep, 2010, 38

(7):4475-4482
[93]Yan J, Cai X, Luo J, Sato S, Jiang Q, Yang J, Cao X, Hu X, Tabata S, Gresshoff PM, Luo D .The REDUCED LEAFLET genes encode key components of the trans-acting small interfering RNA pathway and regulate compound leaf and flower development in Lotus japonicus[J][J].Plant Physiol, 2010, 152

(2):797-807
[94]Yang X, Pang H, Liu B, Qiu Z, Gao Q, Wei L, Dong Y, Wang Y .Evolution of double positive autoregulatory feedback loops in CYCLOIDEA2 clade genes is associated with the origin of floral Zygomorphy[J][J].Plant Cell, 2012, 24

(5):1834-1847
[95]Yu Y, Wang H, Fu Z, Wang J, Liu J .Transcriptional regulation of two RTE-like genes of carnation during flower senescence and upon ethylene exposure, wounding and sucrose[J][J].Plant Biol, 2011, 13

(5):719-724
[96]Zhang J, Wu K, Zeng S, Teixeira da Silva JA, Zhao X, Tian C, Xia H, Duan J .Transcriptome analysis of Cymbidium sinense and its application to the identification of genes associated with floral development[J][J].BMC Genomics, 2013, 14

:279-279
[97]Zhang JD, Cao JP, Qiao AM, He SG, Sun M, Joyce DC.Two phenylalanine ammonia-lyase genes (DcPAL2 and DcPAL3) are involved in the cut-induced stem-end wound reaction of carnation (Dianthus caryophyllus L.)[J][J].J Hort Sci Biotech, 2011, 864

(4):377-383
[98]Zhang J, Li Z, Guo C, Liu G, Bao M .Isolation and functional analys es of a putative floral homeotic C-function gene in a basal eudicot London plane tree (Platanus acerifolia)[J][J]., 2013, 8

(5):-
[99]Zhang L, Li L, Wu J, Peng J, Zhang L, Wang X.Cell expansion and microtubule behavior in ray floret petals of Gerbera hybrida : Responses to light and gibberellic acid[J][J].Photochem Photobiol Sci, 2012, 11

(2):279-288
[100]Zhou X, Wang Y, Smith JF, Chen R.Altered expression patterns of TCP and MYB genes relating to the floral developmental transition from initial zygomorphy to actinomorphy in Bournea (Gesneriaceae)[J][J].New Phytol, 2008, 178

(3):532-543
文章导航

/