研究报告

外源腐胺促进苹果果皮花青苷积累的效应

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  • 中国农业科学院果树研究所国家苹果育种中心, 兴城 125100

收稿日期: 2008-08-14

  修回日期: 2008-12-30

  网络出版日期: 2010-11-03

Promotion Effect of Exogenous Putrescine on Anthocyanin Accumulation in ‘Red Fuji’ Apple Fruits

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  • National Center for Apple Breeding, Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, China

Received date: 2008-08-14

  Revised date: 2008-12-30

  Online published: 2010-11-03

摘要

为了探讨外源施加腐胺对苹果果皮花青苷合成相关基因的调控效应和果实着色的影响, 摘袋当天对苹果品种红富士(Malus domestica Borkh. ‘Red Fuji’)果实喷施50 mg.L-1腐胺(putrescine, Put), 利用分光光度计和高效液相色谱仪分别对苹果果皮花青苷含量及其组成进行了分析; 利用实时荧光定量PCR法检测了转录调节因子MYB1和5个花青苷合成结构基因的转录水平。结果表明: (1) 外源喷施Put对于苹果果皮中花青苷的积累具有明显的促进效应, 在果实采收时, 处理组果皮中的花青苷含量为对照组的1.9倍; (2) 处理果实的果皮中含有矢车菊素阿拉伯糖苷(cyaniding-3-arabinoside, Cy-3-ara), 而在相同条件下, 对照组中未能检测到Cy-3-ara; (3) Put处理对于转录调节因子MYB1和类黄酮3, 5-糖苷转移酶(UDP-glycose: flavonoid 3-O-glycosyltransferase, UFGT)基因的转录有明显的促进作用, 摘袋后第1天和第3天, Put处理组的MYB1转录水平分别为对照组的1.6和2.0倍, UFGT变化趋势与MYB1类似, 查耳酮异构酶(chalcone isomerase, CHI)、花青素苷元还原酶 (anthocyanidin reductase, ANR)和无色花青素加双氧酶(leucoanthocyanidin dioxygenase, LDOX)等基因的转录水平在Put处理初期也表现为明显上升, 特别是 LDOX基因, 其转录水平在处理后第1天和第3天分别达到对照的10.2和3.8倍。在所研究的基因中, 二氢类黄酮还原酶(dihydroflavonol 4-reductase, DFR)基因是唯一一个经Put处理后其转录水平受到强烈抑制的基因, 且这种抑制作用在摘袋后第3天最为明显, 对照组的DFR转录水平为Put处理组的2.3倍。

本文引用格式

田义;王强;张利义;康国栋;杨玲;郝红梅;杨振英;丛佩华* . 外源腐胺促进苹果果皮花青苷积累的效应[J]. 植物学报, 2009 , 44(03) : 310 -316 . DOI: 10.3969/j.issn.1674-3466.2009.03.007

Abstract

In order to explore the effect of exogenous putrescine (Put) on anthocyanin accumulation in apple skin, putrescine (50 mg·L-1) was applied to ‘Red Fuji’apple fruits immediately after bags were removed from fruit. Anthocyanin accumulation was determined by spectrophotometer and high-performance liquid chromatography, and transcriptional levels of several anthocyanin synthesis-related genes including MYB1 and genes encoding UDP-glycose: flavonoid 3-O-glycosyltransferase (UFGT), chalcone isomerase (CHI), anthocyanidin reductase (ANR), leucoanthocyanidin dioxygenase (LDOX) and dihydroflavonol 4-reductase (DFR), were determined by real-time fluorescence quantitative PCR. Anthocyanin content was significantly promoted with Put treatment, to about 1.9-fold that with control treatment. Put treatment revealed cyanidin 3-arabinoside content, although low, which was not revealed with control treatment. The transcriptional level of MYB1 was increased 1.6-fold with 1-day Put treatment and two-fold with 3-day treatment. The changed transcriptional level of UFGT gene was similar to that of MYB1; transcriptional levels of CHI, ANR and LDOX were increased as well. The transcriptional level of LDOX was 10.2- and 3.8-fold after 1- and 3-day Put treatment, respectively. Among six genes investigated in our study DFR was the only gene of which transcriptional level was inhibited with Put treatment. The strong effect of inhibition was revealed with 3-day treatment that the transcriptional level of DFR with control treatment was 2.3-fold of that with Put treatment.

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