以皱皮木瓜(Chaenomeles speciosa)当年生枝条的茎尖和茎段为外植体进行离体培养与快速繁殖。结果表明: 芽萌发及愈伤组织诱导的最佳培养基是MS+2.0 mg.L^-1 6-BA+1.0 mg.L-¹ NAA; 愈伤组织分化最佳培养基是MS+1.0 mg.L^-1 6-BA+0.05 mg.L^-1 NAA; 芽增殖最佳培养基是MS+1.0 mg.L^-1 6-BA+0.05-0.1 mg.L^-1 NAA, 其增殖系数为6.59; 最佳生根培养基是1/2MS+1.0 mg.L^-1 IAA, 生根率为86.7%; 采用珍珠岩二步移栽, 成活率可达90%以上。

This experiment used the current stem-segment with shoot apexes and segments as explants in callus induction and rapid propagation of Chaenomeles speciosa. The optimal medium for bud and callus induction was MS+2.0 mg.L^-1 6-BA+1.0mg. L^-1 NAA, and the optimal propagation medium was MS+1.0 mg.L^-1 6-BA+0.05-0.1 mg.L^-1 NAA. The proliferation coefficient was 6.59. For callus differentiation, the optimal medium was MS+ 1.0 mg.L^-1 6-BA+ 0.05 mg.L^-1 NAA, and for rooting, 1/2 MS+1.0 mg. L^-1 IAA. The ratio of rooting was 86.7%, and that of survival was more than 90% when small intact plants were transplanted on perlite seedbeds.