花青素苷(anthocyanins)成分和含量的分析是花色研究的重要内容之一。该文利用高效液相色谱-电喷雾离子化-质谱技术(HPLC-ESI-MS), 建立了一种快速有效地分析烟草(Nicotiana tabacum)花冠中花青素苷成分及含量的方法。在保证良好分离效果的基础上, 将分析时间缩短至15分钟, 大大提高了分析速度, 降低了成本, 并成功应用于转基因烟草花冠中花青素苷成分的分析。为不具备高效分析仪器(如超高效液相色谱-串联质谱(UPLC-MS/MS))的一般实验室研究花青素苷合成相关基因在烟草中的异源表达提供了一种有效且实用的分析方法。
The analysis of anthocyanins is an important task for studies of flower coloration. This paper describes a rapid, effective method for analysis of anthocyanin composition and content in tobacco corolla based on high-performance liquid chromatography-electrospray ionization-mass spectrometry. HPLC analysis time was shortened to 15 min by this method, and the separation effect was guaranteed. The analysis speed is largely improved, and costs are reduced. The analysis of anthocyanins in transgenic tobacco was successful with this method, so it can be used in a general laboratory, even without advanced instruments such as ultra performance liquid chromatography-mass spectrometry/mass spectrometry. This method offers a practical approach to studies of heterologous expression of anthocyanin biosynthesis pathway- related genes in tobacco.
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