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DOI: https://doi.org/10.11983/CBB25007

贝莱斯芽孢杆菌对芒果炭疽病的抑制作用及果实保鲜效果

  • 曹慧 ,
  • 羊尾燕 ,
  • 纳琦婷 ,
  • 朱长松 ,
  • 宋海超 ,
  • 史学群
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  • 1海南大学食品科学与工程学院, 海口570228; 2海南大学生命健康学院, 海口570228

收稿日期: 2025-01-15

  修回日期: 2025-02-06

  网络出版日期: 2025-02-26

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The Inhibitory Efficacy of Bacillus velezensis Against Mango Anthracnose and Its Influence on Fruit Fresh Preservation

  • CAO Hui ,
  • YANG Wei-Yan ,
  • NA Qi-Ting ,
  • ZHU Zhang-Song ,
  • SONG Hai-Tiao ,
  • SHI Hua-Qun
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  • 1College of Food Science and Engineering, Hainan University, Haikou 570228, China; 2College of Life and Health Sciences, Hainan University, Haikou 570228, China

Received date: 2025-01-15

  Revised date: 2025-02-06

  Online published: 2025-02-26

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摘要

炭疽病是芒果主要病害之一, 是由胶孢炭疽菌(Colletotrichum gloeosporioides)侵染引起。发病后影响果实品质从而缩短果实货架期。该研究从生物防治角度出发, 以贝莱斯芽孢杆菌(Bacillus velezensis)和从芒果果实中分离出的炭疽菌进行实验, 用贝莱斯芽孢杆菌菌悬液(CB)处理芒果果实, 并利用平板对峙法观察CB对胶孢炭疽菌的抑制效果。通过发酵液(CF)处理胶孢炭疽菌, 结果表明发酵液能够有效抑制胶孢炭疽菌的孢子萌发并缩短芽管长度, 显著抑制菌丝生长, 其中发酵液(CF)浓度为2%和4%时, 对炭疽菌菌丝抑制率分别为75.18%和80.96%。果实活体接种实验表明, CB (菌悬液)和CF (发酵液)能有效抑制果实病斑直径的增大, 抑制率分别为44.33%和65.00%; 与对照组相比, 处理组可溶性固形物含量显著降低, 减缓了可滴定酸含量的降低, 对果实品质未造成负面影响; 同时也延缓了果实总酚和类黄酮含量的降低。实验表明, 贝莱斯芽孢杆菌菌株能够抑制芒果炭疽病的发生, 在芒果保鲜方面有较大的应用潜力。

关键词： 芒果; 胶孢炭疽菌; 贝莱斯芽孢杆菌; 生物防治; 果实保鲜

本文引用格式

曹慧 , 羊尾燕 , 纳琦婷 , 朱长松 , 宋海超 , 史学群 . 贝莱斯芽孢杆菌对芒果炭疽病的抑制作用及果实保鲜效果[J]. 植物学报, 0 : 1 -0 . DOI: 10.11983/CBB25007

Abstract

INTRODUCTION: Anthracnose, primarily caused by Colletotrichum gloeosporioides is a main diseaseaffecting mangoes, leading to significant postharvest losses by deteriorating fruit quality and reducing shelf life. 

RATIONALE: Addressing postharvest anthracnose is a critical challenge in the mango industry. Biological control methods, such as utilizing antagonistic bacteria, offer sus-tainable alternatives to chemical treatments. This study investigates the efficacy of Ba-cillus velezensis in inhibiting C. gloeosporioides and its potential in preserving mango fruit quality. 

RESULTS: The application of B. velezensis culture filtrate (CF) effectively inhibited spore germination and mycelial growth of C. gloeosporioides. At CF concentrations of 2% and 4%, mycelial inhibition rates were 75.18% and 80.96%, respectively. In vivo experiments demonstrated that both bacterial suspension (CB) and CF treatments significantly re-duced lesion expansion on mangoes, with inhibition rates of 44.33% and 65.00%, respectively. Treated fruits exhibited a slower decrease in titratable acids and maintained higher levels of total phenols and flavonoids, indicating delayed ripening and extended shelf life. 

 CONCLUSION: Bacillus velezensis exhibits strong antagonistic activity against C. gloeosporioides, effectively controlling mango anthracnose and preserving fruit quality. Its application as a biocontrol agent holds promise for sustainable postharvest management in mango production.

Key words： Mango; Colletotrichum gloeosporioides; Bacillus velezensis; biological control; fresh preservation

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参考文献

褚睿, 李昭轩, 张学青, 杨东亚, 曹行行, 张雪艳(2023). 黄瓜枯萎病拮抗芽孢杆菌的筛选、鉴定及其生防潜力. 生物技术通报 39(08), 262-271. 甘林, 代玉立, 刘晓菲, 兰成忠, 杨秀娟(2024). 香蕉枯萎病高效拮抗土著细菌的筛选及其防效. 西北农林科技大学学报(自然科学版) 52(06), 95-105. 高爱平, 陈业渊, 朱敏, 何业华, 杜中军(2006). 中国芒果科研进展综述. 中国热带农业 (6), 21-23. 姜爱丽, 胡文忠, 李慧, 田密霞, 范圣第(2009). 纳他霉素处理对采后甜樱桃生理代谢及品质的影响. 农业工程学报 25(12), 351-356. 李其利, 卜俊燕, 唐利华, 黄穗萍, 郭堂勋, 莫贱友(2020). 芒果炭疽菌研究进展. 微生物学杂志 40(01), 117-124. 梁松, 孟祥坤, 于新, 徐文凤, 邢芳芳, 郑树林, 李新柱, 王亮(2023). 草莓叶枯病生防芽孢杆菌的分离鉴定及防效研究. 河南农业科学 52(10), 92-99. 皮娜娜, 王玺茜, 罗建军, 翁群芳(2024). 贝莱斯芽孢杆菌防控植物病害的研究进展. 广东农业科学 51(06), 48-59. 田中欢, 杜玉杰, 卢永清, 纪思蓉, 龙超安(2023). 贝莱斯芽孢杆菌w176对柑橘采后青霉病的防治. 中国植物病理学会2023年学术年会论文集. 北京: 中国植物病理学会. 2023. 637. 王文静, 罗睿雄, 黄建峰, 赵志常, 高爱平(2020). 杧果炭疽病研究进展. 云南农业科技 (06), 42-45. 许家辉, 余东, 许玲, 刘惠玉(2007). 福建芒果产业状况及发展对策. 福建果树 (03), 21-22. 杨建波, 欧阳秋飞, 黄战威(2019). 杧果采后主要真菌病害及其抗病机理研究进展. 热带农业科学 39(10), 106-110. 杨可, 司文, 林海, 林方锐, 袁静, 陈杰(2019). 利用响应面分析法优化贝莱斯芽孢杆菌TCS001的发酵条件. 农药学学报 21(04), 444-452. 杨可, 郑柯斌, 黄晓慧, 袁静, 陈杰(2018). 海洋生境贝莱斯芽孢杆菌TCS001的鉴定及抑真菌活性. 农药学学报 20(03), 333-339. 杨林杰(2023). 负载肉桂精油Pickering乳液活性膜的构建及保鲜应用研究. 华中农业大学. 杨然迪, 杨怡妍, 曹灏, 金京, 陈杰(2024). 贝莱斯芽孢杆菌TCS001产脂肽类物质抑菌活性及发酵条件优化. 农药学学报 26(03), 504-516. 杨芝霓, 鲁萌萌, 黄穗萍, 唐利华, 陈小林, 郭堂勋, 莫贱友, 张萌, 李其利(2023). 产挥发性物质芽孢杆菌对芒果炭疽菌的抑制作用及对芒果炭疽病的防病效果. 植物病理学报 53(06), 1180-1191. 詹儒林, 李伟, 郑服丛(2005). 芒果炭疽病菌对多菌灵的抗药性. 植物保护学报 (01), 71-76. 张晓勇, 李树江, 严凯, 翁贵英, 张韵霞, 龙巧芳, 梁文, 蒋芹娜, 杨友联(2021). 杧果采后炭疽病生防菌株筛选及其培养特性研究. 园艺学报 48(11), 2171-2184. 章乐乐, 王冠, 柳凤, 胡汉桥, 任磊(2023). 芒果炭疽病拮抗菌分离、鉴定及生防机制研究. 生物技术通报 39(04), 277-287. 赵焕兰(2022). 贝莱斯芽孢杆菌对樱桃番茄采后灰霉病的抑制机理研究及保鲜效果评价. 合肥工业大学. 赵佳, 童凯, 李东, 邓孟胜, 雷雨(2022). 芒果采后炭疽病生防菌研究进展. 保鲜与加工, 22(08), 88-96. 赵鲁宁, 周秋阳, 杨慧慧, 郑鸿儒, 温新宇, 孙卫红(2019). 季也蒙毕赤酵母Y35-1菌株对枇杷采后炭疽病的抑菌效果及保鲜作用. 食品科学 40(04), 170-177. Chanchaichaovivat A, Ruenwongsa P, Panijpan B(2007). Screening and identification of yeast strains from fruits and vegetables: Potential for biological control of postharvest chilli anthracnose (Colletotrichum capsici). Biological Control 42(3), 326-335. Choub V, Maung CEH, Won SJ, Moon JH, Kim KY, Han YS, Cho JY, Ahn YS(2021). Antifungal activity of cyclic tetrapeptide from Bacillus velezensis CE 100 against plant pathogen Colletotrichum gloeosporioides. Pathogens 10(2), 209. Dofuor AK, Quartey NKA, Osabutey AF, Antwi-Agyakwa AK, Asante K, Boateng BO, Ablormeti FK, Lutuf H, Osei-Owusu J, Osei JHN, Ekloh W, Loh SK, Honger JO, Aidoo OF, Ninsin KD(2023). Mango anthracnose disease: the current situation and direction for future research. Frontiers in Microbiology 14, 1168203. Droby S, Wisniewski ME, Cohen L, Weiss B, Touitou D, Eilam Y, Chalutz E(2007). Influence of CaCl2 on Penicillium digitatum, grapefruit peel tissue, and biocontrol activity of Pichia guilliermondii. Phytopathology 87(3), 310-315. Li SP, Xiao QL, Yang HJ, Huang JG, Li Y(2022). Characterization of a new Bacillus velezensis as a powerful biocontrol agent against tomato gray mold. Pesticide Biochemistry and Physiology 187, 105199. Lima G, De Curtis F, Castoria R, De Cicco V(1998). Activity of the yeasts Cryptococcus laurentii and Rhodotorula glutinis against post-harvest rots on different fruits. Biocontrol Science and Technology 8(2), 257-267. Miguel MG, Gago C, Antunes MD, Lagoas S, Faleiro ML, Megías C, Cortés-Giraldo I, Vioque J, Figueiredo AC(2018). Antibacterial, antioxidant, and antiproliferative activities of Corymbia citriodora and the essential oils of eight Eucalyptus species. Medicines 5(3), 61. Pal, RK(1998). Ripening and rheological properties of mango as influenced by ethrel and calcium carbide. 358-360. Wang L, Zhu T(2023). Strong opponent of walnut anthracnose Bacillus velezensis and its transcriptome analysis. Microorganism. 11 (8), 1-22.
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