大豆TPS基因家族在盐胁迫下的表达变化及单倍型选择规律分析(长英文摘要)
†共同第一作者
收稿日期: 2024-07-22
录用日期: 2024-12-14
网络出版日期: 2024-12-27
基金资助
广东省自然科学基金面上项目(2023A1515011668);黑龙江省自然科学基金(LH2021C078);河北省现代种业科技创新专项(22326316D)
Changes in the Expression of the Soybean TPS Gene Family Under Salt Stress and Haplotype Selection Analysis
†These authors contributed equally to this paper
Received date: 2024-07-22
Accepted date: 2024-12-14
Online published: 2024-12-27
海藻糖-6-磷酸合酶(trehalose-6-phosphate synthase, TPS)是合成海藻糖的关键酶, 已在多个物种中被报道参与调节光合作用、糖代谢、生长发育和逆境响应等生理过程。目前, TPS在大豆(Glycine max)中的报道极少。该文在大豆全基因组中鉴定了20个TPS基因及其包含的10种重要蛋白保守基序。启动子元件分析显示, 大豆TPS基因的启动子区富含大量胁迫响应元件; 盐胁迫处理后, 17个TPS基因的表达发生变化, 其中12个基因上调表达, 5个基因下调表达。对TPS进行单倍型和选择趋势分析, 发现TPS8、TPS13、TPS15、TPS17和TPS18存在2种主要的等位变异, 其中携带TPS15H2、TPS13H2、TPS17H2和TPS18H2的品种在栽培品种中大量富集, 受到强烈的人工选择。该研究揭示了大豆TPS基因家族的分子特征以及在盐胁迫下的表达模式和进化历史, 旨在为进一步解析大豆TPS基因的功能以及培育耐盐大豆品种提供理论依据和遗传材料。
关键词： 大豆; 海藻糖-6-磷酸合酶; 表达分析; 耐盐; 人工选择
曹婕 , 卢秋连 , 翟健平 , 刘宝辉 , 方超 , 李世晨 , 苏彤 . 大豆TPS基因家族在盐胁迫下的表达变化及单倍型选择规律分析(长英文摘要)[J]. 植物学报, 2025 , 60(2) : 172 -185 . DOI: 10.11983/CBB24110
INTRODUCTION Trehalose-6-phosphate synthase (TPS) is a key enzyme involved in the synthesis of trehalose and has been reported to participate in regulating photosynthesis, carbohydrate metabolism, growth and development, and stress responses in various species. Currently, reports on TPS genes in soybean are scarce.
RATIONALE TPS is a stable non-reducing disaccharide, whose synthesis, decomposition and regulation not only provide energy for plant, but also play an important role in plant growth and development and stress tolerance. The in-depth study of soybean TPS genes and its relationships with salt stress is of great significance in elucidating the molecular mechanism of soybean salt tolerance and improving soybean yield.
RESULTS This study identified 20 soybean TPS genes and their associated 10 conserved protein motifs in the soybean genome. Molecular analysis of the promoter elements revealed that the TPS gene promoters are rich in stress-responsive elements. After salt stress treatment, the expression of 17 TPS genes changed, with 12 genes up-regulated and 5 genes down-regulated. Haplotype and selection analyses revealed two major allelic variations in TPS8, TPS13, TPS15, TPS17, and TPS18. Notably, variants carrying TPS15H2, TPS13H2, TPS17H2, and TPS18H2 were significantly enriched in improved cultivars that underwent strong artificial selection.
CONCLUSION This study reveals the molecular characteristics of the soybean TPS gene family, their expression patterns under salt stress, and their evolutionary history, providing a theoretical basis and genetic material for further elucidating the functions of soybean TPS genes and breeding salt-tolerant soybean varieties.
TPS genes were subjected to intense artificial selection. The natural variations of TPS8, TPS13, TPS15, TPS17, and TPS18 have been subjected to strong artificial selection during soybean domestication and improvement, with the variants carrying TPS15H2, TPS13H2, TPS17H2, and TPS18H2 being heavily enriched in improved cultivars.
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